PXD046313
PXD046313 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Enhanced removal of Cd2+ from water by a bacterioferritin from Candidatus Kuenenia stuttgartiensis within engineering bacteria |
| Description | Cadmium can enter water through various industrial pollutants, posing serious threats to the ecological environment and human health. This study unveiled an effective method for removing Cd2+ from water by introducing bacterioferritin, known as Candidatus Kuenenia stuttgartiensis (K.S Bfr), into engineering bacteria (EB) to facilitate smetal ion removal via K.S Bfr storage. Additionally, the paper explores the mechanism behind Cd2+ removal by EB using K.S Bfr. The binding kinetics revealed that EB’s Cd2+ binding capacity was significantly higher than that of wild bacteria, and Cd2+ was found to enter the protein cavity of K.S Bfr in vitro. When exposed to Cd2+ concentrations ranging from 0 to 2.5 mM, EB demonstrated a survival rate above 70%, whereas wild bacteria's survival rate remained below 50%. The superior Cd2+ removal capacity of EB can be attributed to the intracellular storage capacity of K.S Bfr. Tandem Mass Tags quantitative proteomics analysis showed that EB mitigated cytotoxicity mainly by up-regulating the expression of binding, homeostasis, efflux, and ribosome-related proteins. K.S Bfr was identified as a major pathway for bacteria to remove Cd2+ since it provided independent storage space for Cd2+ and ensured intracellular metal ion homeostasis. This study presents an effective strategy for treating metal wastewater, offering promising new applications for bioremediation. |
| HostingRepository | iProX |
| AnnounceDate | 2023-10-23 |
| AnnouncementXML | Submission_2023-10-22_23:56:50.480.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jin Wang |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-22 23:56:25 | ID requested | |
| ⏵ 1 | 2023-10-22 23:56:50 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Cd2+ removal, Bacterioferritin, Bioremediation, Proteomics |
Contact List
| Youpeng Chen | |
|---|---|
| contact affiliation | Chongqing University |
| contact email | ypchen@cqu.edu.cn |
| lab head | |
| Jin Wang | |
| contact affiliation | Chongqing University |
| contact email | jinwangjf@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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