PXD046301

PXD046301 is an original dataset announced via ProteomeXchange.

Title	A study of the mechanism whereby Au-Ag-PDA@MSCM-nanoparticle-mediated photothermal therapy induces sebaceous gland ferroptosis during the treatment of acne
Description	Branched gold and silver nanoparticles with a polydopamine coating (Au-Ag-PDA) are promising nanomaterials with high photothermal conversion efficiency. Previous studies have shown that a low concentration of Au-Ag-PDA nanoparticles coated with mesenchymal stem cell membranes (Au-Ag-PDA@MSCM), in conjunction with 808 nm LASER irradiation, can be used to treat acne by shrinking the sebaceous gland tissue to inhibit sebum secretion. However, the exact mechanism underlying this treatment remains unclear. We systematically studied the mechanism by which Au-Ag-PDA@MSCM-mediated photothermal therapy (PTT) inhibits sebum secretion in vitro and vivo, using proteomic analyses, biochemical indicators of ferroptosis, bio-transmission electron microscopy, and western blotting assays. To the best of our knowledge, we revealed for the first time that Au-Ag-PDA@MSCM-mediated PTT induced early ferroptosis by upregulation acyl-coenzyme A synthetase long-chain family member 4 (ACSL4) in sebaceous gland cells, including features such as mitochondrial contraction, increased membrane density. sebaceous gland cells iron deposition, lipid peroxidation, and glutathione (GSH) depletion. Using proteomics, we also found that the sebaceous gland cells had mitochondrial respiratory chains and redox imbalances after Au-Ag-PDA@MSCM-mediated PTT. We also found that the Hippo signaling pathway was enriched after this procedure. The results of this study will aid our understanding of how Au-Ag-PDA@MSCM-mediated PTT inhibits sebum secretion and treats acne. Our study also provides an important theoretical basis for studying the mechanisms involved in the use of nanomaterials for photothermal treatment. Sample processing protocol（组学相关的实验材料方法；字数要求500-5000）
HostingRepository	iProX
AnnounceDate	2023-10-23
AnnouncementXML	Submission_2023-10-22_20:46:16.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mingji Zhu
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-22 20:45:08	ID requested
⏵ 1	2023-10-22 20:46:17	announced

Dataset with its publication pending

submitter keyword: Proteomics, Au-Ag-PDA@MSCM, photothermal therapy, ferroptosis, acne, Acsl4

Mingji Zhu
contact affiliation	China-Japan Union Hospital of Jilin University
contact email	zmj@jlu.edu.cn
lab head
Mingji Zhu
contact affiliation	China-Japan Union Hospital of Jilin University
contact email	zmj@jlu.edu.cn
dataset submitter

iProX dataset URI