PXD046292

PXD046292 is an original dataset announced via ProteomeXchange.

Title	Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases
Description	The study is first to describe the temporal and differential transcriptional expression of two lytic polysaccharide monooxygenase (LPMO) genes of Rasamsonia emersonii in response to various carbon sources. The mass spectrometry based expression of carbohydrate active enzymes (CAZymes) on different carbon sources showed varying levels of LPMOs (AA9), AA3, AA7, catalase, and superoxide dismutase pointing to the redox interplay between the LPMOs and auxiliary enzymes. Moreover, it was observed that cello-oligosaccharides have a negative impact on the expression of LPMOs, which has not been highlighted in any previous research. The LPMO1 (30 kDa) and LPMO2 (47 kDa), cloned and expressed in Pichia pastoris were catalytically active with (Kcat/Km) of 6.6 × 10-2 mg-1 ml min-1 and 1.8 × 10-2 mg-1 ml min-1 against Avicel, respectively. The mass spectrometry of hydrolysis products of Avicel/CMC showed presence of C1/C4 oxidized oligosaccharides indicating them to be type 3 LPMOs. The 3D structural analysis of LPMO1 and LPMO2 revealed distinct arrangement of conserved catalytic residues. Furthermore, the developed enzyme cocktails consisting of cellulase from R. emersonii mutant M36 supplemented with recombinant LPMO1/LPMO2 resulted in significantly enhanced saccharification of steam/acid pretreated unwashed rice straw slurry from PRAJ industries (Pune, India). The current work indicates that LPMO1 and LPMO2 are catalytically efficient and have a high degree of thermostability, emphasising their usefulness in improving benchmark enzyme cocktail performance.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:44:32.814.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046292
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marcos Di Falco
SpeciesList	scientific name: Fungi; NCBI TaxID: NCBITaxon:4751; scientific name: Rasamsonia emersonii; NCBI TaxID: 68825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-22 18:06:47	ID requested
⏵ 1	2024-10-17 02:44:33	announced

10.6019/PXD046292;

10.1007/s00253-024-13240-0;

Raheja Y, Singh V, Kumar N, Agrawal D, Sharma G, Di Falco M, Tsang A, Chadha BS, Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases. Appl Microbiol Biotechnol, 108(1):444(2024) [pubmed]

submitter keyword: LC-MSMS, Rasamsonia emersonii,polysaccharide monooxygenase

Bhupinder Singh Chadha
contact affiliation	Department of Microbiology Guru Nanak Dev University Amritsar 143005 Punjab India
contact email	chadhabs@yahoo.com
lab head
Marcos Di Falco
contact affiliation	Concordia University
contact email	marcos.difalco@concordia.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046292
     1. Label: PRIDE project
     2. Name: Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases