PXD046288

PXD046288 is an original dataset announced via ProteomeXchange.

Title	Protein profiling of plasma samples of patients with dilated cardiomyopathy and differences according to the disease prognosis
Description	Dilated cardiomyopathy (DCM) is a disease of the heart muscle characterized by left ventricular or biventricular dilation and systolic dysfunction. It presents one of the most common causes of heart failure worldwide and also the most common indication for heart transplantation. Despite the discharge treatment possibilities, the outcome of patients with recent onset DCM often remains unpredictable and major adverse events may occur in the first months following the diagnosis. Enhancement in the left ventricular ejection fraction – designed as the left ventricular reverse remodeling (LVRR) – has been considered one of the most important determinants of the improvement and treatment response in the prognosis of DCM. In the present study we performed a comprehensive quantitative proteomic analysis of DCM patients’ plasma samples. First we searched for the differences against healthy control group and found several proteins and biological processes to be significantly regulated. The most DCM up-regulated terms corresponded to inflammatory response, wound healing and complement. Among the most relevant DCM up-regulated proteins were CRP, CD163, AOC3, PF4, IGF2, IGFBP2 and TNC. Down-regulation in DCM samples was observed in the biological processes of blood coagulation and lipid metabolism, both of which present risk factors in cardiovascular diseases. The most relevant down-regulated proteins in DCM were TTN, GSN, FCN3, PON1, PON3, and COMP. In the second part of the study, we observed the clinical fate of the DCM patients one year after admission. We divided them according to their treatment response into subgroups of responders (LVRR+) and non-responders (LVRR-) and searched for proteins that would help to predict the patient’s fate or to elucidate the molecular consequences of the disease progression. The protein with the best prognostic potential was ALDOB, its elevated plasma level was found in patients with worse or fatal outcome. The patients with no or poor treatment response had also up-regulated immunoglobulins and pathways corresponding to immunoglobulin-mediated immune response together with cell adhesion which points to the importance of these processes in the DCM pathology.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:01.793.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046288
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jana Klimentova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-22 15:33:45	ID requested
1	2024-02-19 03:26:26	announced
⏵ 2	2024-10-22 06:31:02	announced	2024-10-22: Updated project metadata.

10.6019/PXD046288;

Klimentova J, Rehulka P, Stulik J, Vozandychova V, Rehulkova H, Jurcova I, Lazarova M, Aiglova R, Dokoupil J, Hrecko J, Pudil R, Searching for Biomarkers with Potential to Predict the Outcome of Therapy. J Proteome Res, 23(3):971-984(2024) [pubmed]

10.1021/acs.jproteome.3c00691;

submitter keyword: left ventricular reverse remodeling, LFQ, functional enrichment analysis, plasma proteome profiling,dilated cardiomyopathy, LC-MS/MS

Jiří Stulík
contact affiliation	Department of Molecular Pathology and Biology, Faculty of Military Health Sciences
contact email	jiri.stulik@unob.cz
lab head
Jana Klimentova
contact affiliation	Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
contact email	jana.klimentova@unob.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046288

PRIDE project URI

• PRIDE
  1. PXD046288
     1. Label: PRIDE project
     2. Name: Protein profiling of plasma samples of patients with dilated cardiomyopathy and differences according to the disease prognosis