PXD046280

PXD046280 is an original dataset announced via ProteomeXchange.

Title	Cell membrane proteome analysis in HEK293T cells challenged with α-Synuclein amyloids
Description	Amyloids interact with plasma membranes. Extracellular amyloids cross the plasma membrane barrier. Internalized extracellular amyloids are reported to trigger amyloidogenesis of endogenous proteins in recipient cells. To what extent these extracellular and intracellular amyloids perturb the plasma membrane proteome is not investigated. Using α-Synuclein as a model amyloid protein, we performed membrane shaving followed by mass spectrometry experiment to identify the conformational changes in the cell surface proteins after extracellular amyloid challenge. We also performed membrane proteomics after the biogenesis of intracellular α-Synuclein amyloids. Our results suggest that promiscuous interaction with extracellular amyloids stochastically alter the conformation of plasma membrane proteins. This affects the biological process through the plasma membrane and result in loss in cell viability. Cells that survive the extracellular amyloid shock can grow normally and gradually develop intracellular amyloids which do not directly impact the plasma membrane proteome and associated biological processes. Thus, α-Synuclein amyloids can damage the plasma membrane and related processes only during cell to cell transfer and not during their intracellular biogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-07-09
AnnouncementXML	Submission_2024-07-09_00:10:07.594.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046280
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Swasti Raychaudhuri
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-21 07:07:41	ID requested
⏵ 1	2024-07-09 00:10:08	announced

10.1007/S12038-024-00457-4;

10.6019/PXD046280;

submitter keyword: membrane shaving, internalization,α-synuclein, plasma membrane, amyloids

Swasti Raychaudhuri
contact affiliation	Principal Scientist, CSIR- Centre for Cellular and Molecular Biology, Hyderabad, India
contact email	rcswasti@ccmb.res.in
lab head
Swasti Raychaudhuri
contact affiliation	CSIR-Centre for Cellular and Molecular Biology
contact email	rcswasti@ccmb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046280
     1. Label: PRIDE project
     2. Name: Cell membrane proteome analysis in HEK293T cells challenged with α-Synuclein amyloids