PXD046228

PXD046228 is an original dataset announced via ProteomeXchange.

Title	Mirror-image trypsin digestion and sequencing of D-proteins
Description	The development of mirror-image biology systems and related applications is hindered by the lack of effective methods to sequence mirror-image (D-) proteins. Although natural-chirality (L-) proteins can be sequenced by bottom–up liquid chromatography–tandem mass spectrometry (LC–MS/MS), the sequencing of long D-peptides and D-proteins with the same strategy requires digestion by a site-specific D-protease before mass analysis. Here we apply solid-phase peptide synthesis and native chemical ligation to chemically synthesize a mirror-image version of trypsin, a widely used protease for site-specific protein digestion. Using mirror-image trypsin digestion and LC–MS/MS, we sequence a mirror-image large subunit ribosomal protein (L25) and a mirror-image Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), and distinguish between different mutants of D-Dpo4. We also perform writing and reading of digital information in a long D-peptide of 50 amino acids. Thus, mirror-image trypsin digestion in conjunction with LC–MS/MS may facilitate practical applications of D-peptides and D-proteins as potential therapeutic and informational tools.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:24:02.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guanwei Zhang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-18 22:51:11	ID requested
1	2024-01-19 04:56:57	announced
⏵ 2	2024-10-22 06:24:02	announced	2024-10-22: Updated project metadata.

10.1038/S41557-023-01411-X;

submitter keyword: Total chemical synthesis, D-Trypsin, D-protein sequencing,Mirror-image biology, LC-MS/MS

Ting Zhu
contact affiliation	School of Life Sciences, Westlake University
contact email	tzhu@westlake.edu.cn
lab head
Guanwei Zhang
contact affiliation	School of Life Sciences, Westlake University
contact email	zgw18@mails.tsinghua.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046228

PRIDE project URI

• PRIDE
  1. PXD046228
     1. Label: PRIDE project
     2. Name: Mirror-image trypsin digestion and sequencing of D-proteins