PXD046212

PXD046212 is an original dataset announced via ProteomeXchange.

Title	Endolysosomal TRPML1 channel regulates cancer cell-migration by facilitating the intracellular trafficking of E-cadherin and β1-integrin
Description	Recently, endolysosomal cation channels have emerged as an attractive anti-cancer target: namely the mucolipin subfamily of transient potential receptors (TRPMLs), which comprises three isoforms – TRPML1 (MCOLN1), TRPML2 (MCOLN2), and TRPML3 (MCOLN3). TRPML1, the most intensively researched member of the family, is ubiquitously expressed in the membranes of endosomes and lysosomes, whereas TRPML2 and TRPML3 are mainly localized in specialized cells (e. g. immune cells, hair cells of the inner ear, secretory cells, and melanocytes). In past research TRPML1 has been linked to ion homeostasis, vesicular trafficking, and autophagy. Aside from these physiological functionalities, TRPML1’s role in cancer is emerging. Interestingly, it has been implicated to regulate cancer cell migration as its inhibition reduces invasiveness of breast cancer cells in vitro and in vivo. However, the underlying mechanism is still vastly elusive. Given the apparent correlation between TRPML1 and cancer cell migration, we aimed to further elucidate its role in cancer cell migration in hepatocellular carcinoma and, most importantly, uncover the underlying mechanisms by monitoring cell-junctional and cell-adhesion proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:34:10.108.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Bernd Stöckl
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-18 08:24:15	ID requested
1	2024-03-20 09:09:58	announced
⏵ 2	2024-10-22 06:34:11	announced	2024-10-22: Updated project metadata.

10.1016/j.jbc.2023.105581;

Frey N, Ouologuem L, Blenninger J, Siow WX, Thorn-Seshold J, St, ö, ckl J, Abrahamian C, Fr, ö, hlich T, Vollmar AM, Grimm C, Bartel K, -integrin. J Biol Chem, 300(1):105581(2024) [pubmed]

submitter keyword: Cancer biology, lysosome, adhesion, ion channel, migration

Thomas Fröhlich
contact affiliation	Ludwig-Maximilians-Universität München Genzentrum Laboratory for Functional Genome Analysis Feodor-Lynen-Str. 25 D-81377 München
contact email	frohlich@genzentrum.lmu.de
lab head
Jan Bernd Stöckl
contact affiliation	Genzentrum der LMU München
contact email	stoeckl@genzentrum.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD046212

PRIDE project URI

• PRIDE
  1. PXD046212
     1. Label: PRIDE project
     2. Name: Endolysosomal TRPML1 channel regulates cancer cell-migration by facilitating the intracellular trafficking of E-cadherin and β1-integrin