PXD046189

PXD046189 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Feasibility of phosphoproteomics to uncover oncogenic signalling in secreted extracellular vesicles using glioblastoma-EGFRVIII cells as a model
Description	Cancer cells secrete extracellular vesicles (EVs) that contain molecular information, including proteins and RNA. Oncogenic signalling can be transferred via the cargo of EVs to recipient cells and may influence the behaviour of neighbouring cells or cells at a distance. This cargo may contain cancer drivers, such as EGFR, and also phosphorylated (activated) components of oncogenic signalling cascades. Till date, the cancer EV phosphoproteome has not been studied in great detail. In the present study, we used U87 and U87EGFRvIII cells as a model to explore EV oncogenic signalling components in comparison to the cellular profile. EVs were isolated using the VN96 ME-kit and subjected to LC-MS/MS based phosphoproteomics and dedicated bioinformatics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:06:08.859.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thang Pham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-17 09:25:30	ID requested
1	2023-10-17 12:00:22	announced
2	2023-11-14 09:06:13	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:06:09	announced	2024-10-22: Updated project metadata.

Publication List

10.1016/j.jprot.2020.104076;
Bijnsdorp IV, Schelfhorst T, Luinenburg M, Rolfs F, Piersma SR, de Haas RR, Pham TV, Jimenez CR, Feasibility of phosphoproteomics to uncover oncogenic signalling in secreted extracellular vesicles using glioblastoma-EGFRVIII cells as a model. J Proteomics, 232():104076(2021) [pubmed]

10.1016/j.jprot.2020.104076;

Bijnsdorp IV, Schelfhorst T, Luinenburg M, Rolfs F, Piersma SR, de Haas RR, Pham TV, Jimenez CR, Feasibility of phosphoproteomics to uncover oncogenic signalling in secreted extracellular vesicles using glioblastoma-EGFRVIII cells as a model. J Proteomics, 232():104076(2021) [pubmed]

Keyword List

submitter keyword: exosome, Phospho-proteomics,Glioblastoma multiforme, EGFR, INKA, LC-MS/MS, Extracellular vesicles

submitter keyword: exosome, Phospho-proteomics,Glioblastoma multiforme, EGFR, INKA, LC-MS/MS, Extracellular vesicles

Contact List

Connie Jimenez
contact affiliation	Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Oncology, Cancer Center Amsterdam, OncoProteomics Laboratory, Amsterdam, Netherlands
contact email	c.jimenez@amsterdamumc.nl
lab head
Thang Pham
contact affiliation	Amsterdam UMC
contact email	t.pham@amsterdamumc.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD046189
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD046189

PRIDE project URI

Repository Record List

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