PXD046178

PXD046178 is an original dataset announced via ProteomeXchange.

Title	Identification of sialyl Lewisa/x-modified surface proteins in Multiple Myeloma cells by LC-MS
Description	The project aims at identifying surface proteins that are modified by the tetrasaccharide sialyl Lewisa/x and therefore serve as selectin ligands in Multiple Myeloma. To that end, we have performed an immunoprecipitation using the Heca452 antibody (which recognizes sialyl Lewisa/x-related structures) and a matched isotype control on membrane-enriched fractions that have been generated from the sialyl Lewisa/x-enriched and parental Multiple Myeloma cell line MM1S and RPMI8226. Using this approach, we have been able to identify candidate proteins that may function as selectin ligands in myeloma.
HostingRepository	PRIDE
AnnounceDate	2024-01-23
AnnouncementXML	Submission_2024-01-23_06:33:24.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Natoni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-17 01:26:55	ID requested
⏵ 1	2024-01-23 06:33:25	announced

Dataset with its publication pending

submitter keyword: Mulriple Myeloma, sialyl lewis a/x, selectin ligands

Alessandro Natoni
contact affiliation	Hematology, Department of Translational and Precision Medicine, Sapienza University, Rome, Italy.
contact email	alessandro.natoni@uniroma1.it
lab head
Alessandro Natoni
contact affiliation	Hematology, Department of Translational and Precision Medicine, Sapienza University, Rome
contact email	alessandro.natoni@uniroma1.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046178

PRIDE project URI

• PRIDE
  1. PXD046178
     1. Label: PRIDE project
     2. Name: Identification of sialyl Lewisa/x-modified surface proteins in Multiple Myeloma cells by LC-MS