PXD046157 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanical control of the splicing regulator PTBP1 regulates extracellular matrix stiffness-induced proliferation and mechanomemory |
Description | Cells sense and respond to mechanical cues from their environment. Mechanical cues have been shown to be important for many biological processes, including embryonic development, aging, cellular homeostasis, and diseases. Cells translate mechanical cues into cellular biochemical signals that govern cellular behaviour like proliferation or migration, via a process called mechanotransduction. However this process and the proteins involved remain incompletely understood. Here, we present an unbiased and large scale screen approach to identify proteins involved in mechanotransduction. The screen revealed that the splicing factor PTBP1 is a novel mechanotransducer. We show that the nuclear localisation of PTBP1 depends on the extracellular matrix stiffness, cell density, and the actomyosin-based contractility of the cell. Furthermore, we demonstrate that PTBP1 promotes the mechanosensitive splicing of the adapter protein Numb, and that alternative splicing of Numb is crucial for matrix stiffness-induced proliferation. Our results support the idea that changes in alternative splicing are an integral part of mechanotransduction and provide a mechanism by which matrix stiffness regulates cell proliferation and the formation of a mechanomemory in cells. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-31 |
AnnouncementXML | Submission_2025-03-31_03:26:22.182.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mark Collins |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-16 03:59:09 | ID requested | |
⏵ 1 | 2025-03-31 03:26:22 | announced | |
Publication List
Keyword List
submitter keyword: PTBP1, mechanotransduction, splicing, extracellular matrix |
Contact List
Mark Collins |
contact affiliation | School of Biosciences University of Sheffield Sheffield, S10 2TN United Kingdom |
contact email | mark.collins@sheffield.ac.uk |
lab head | |
Mark Collins |
contact affiliation | University of Sheffield |
contact email | mark.collins@sheffield.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD046157 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046157
- Label: PRIDE project
- Name: Mechanical control of the splicing regulator PTBP1 regulates extracellular matrix stiffness-induced proliferation and mechanomemory