⮝ Full datasets listing

PXD046157

PXD046157 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMechanical control of the splicing regulator PTBP1 regulates extracellular matrix stiffness-induced proliferation and mechanomemory
DescriptionCells sense and respond to mechanical cues from their environment. Mechanical cues have been shown to be important for many biological processes, including embryonic development, aging, cellular homeostasis, and diseases. Cells translate mechanical cues into cellular biochemical signals that govern cellular behaviour like proliferation or migration, via a process called mechanotransduction. However this process and the proteins involved remain incompletely understood. Here, we present an unbiased and large scale screen approach to identify proteins involved in mechanotransduction. The screen revealed that the splicing factor PTBP1 is a novel mechanotransducer. We show that the nuclear localisation of PTBP1 depends on the extracellular matrix stiffness, cell density, and the actomyosin-based contractility of the cell. Furthermore, we demonstrate that PTBP1 promotes the mechanosensitive splicing of the adapter protein Numb, and that alternative splicing of Numb is crucial for matrix stiffness-induced proliferation. Our results support the idea that changes in alternative splicing are an integral part of mechanotransduction and provide a mechanism by which matrix stiffness regulates cell proliferation and the formation of a mechanomemory in cells.
HostingRepositoryPRIDE
AnnounceDate2025-03-31
AnnouncementXMLSubmission_2025-03-31_03:26:22.182.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMark Collins
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-16 03:59:09ID requested
12025-03-31 03:26:22announced
Publication List
10.1016/J.ISCI.2025.112273;
Keyword List
submitter keyword: PTBP1, mechanotransduction, splicing, extracellular matrix
Contact List
Mark Collins
contact affiliationSchool of Biosciences University of Sheffield Sheffield, S10 2TN United Kingdom
contact emailmark.collins@sheffield.ac.uk
lab head
Mark Collins
contact affiliationUniversity of Sheffield
contact emailmark.collins@sheffield.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD046157
PRIDE project URI
Repository Record List
[ + ]