PXD046148
PXD046148 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic data of PaoP5 or PaoP5(dap1 mutant) infecting PAO1 or PAO1(lon mutant) Genome sequencing and assembly 1 |
| Description | 5mL PAO1 culture was mixed with PaoP5(dap1 mutant) or PaoP5 (MOI=10) for 10 min, and 5mL PAO1(lon mutant) was infected with aoP5(dap1 mutant) (MOI=10) for 10 min. The 1ml sample was then made into pellets. proteomic analysis was performed by Applied Protein Technologies. Biological replication tests were performed 3 times in each group. The samples were lysed with SDT buffer and quantified with BCA protein assay kit. The protein is digested with trypsin and then desalted with a C18 cartridge. The digested peptides were concentrated by vacuum centrifuge, recombined with formic acid, and then analyzed by LC-MS/MS. The peptides were uploaded to a reversed-phase column and connected to a C18 reversed-phase analysis column of formic acid, separated by a linear gradient buffer containing acetonitrile and formic acid at a flow rate of 300 nL/min. The mass spectrometer operates in positive ion mode. The original MS data of each sample were combined, and MaxQuant 1.5.3.17 software was used for identification and quant |
| HostingRepository | iProX |
| AnnounceDate | 2023-10-16 |
| AnnouncementXML | Submission_2023-10-15_20:42:29.036.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leilei Wei |
| SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-15 20:42:20 | ID requested | |
| ⏵ 1 | 2023-10-15 20:42:29 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Proteomic phage |
Contact List
| Haihua Liang | |
|---|---|
| contact affiliation | Southern University of Science and Technology |
| contact email | lianghh@sustech.edu.cn |
| lab head | |
| Leilei Wei | |
| contact affiliation | Northwest University |
| contact email | 2585572972@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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