PXD046120

PXD046120 is an original dataset announced via ProteomeXchange.

Title	The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis
Description	Spermiogenesis defines the final phase of male germ cell differentiation. Multiple deubiquitinating enzymes have been linked to spermiogenesis, yet the impacts of deubiquitination on spermiogenesis remain poorly characterized. Here, we investigated the function of UAF1 in mouse spermiogenesis and male fertility. We selectively deleted Uaf1 in premeiotic germ cells using Stra8-Cre knock-in mouse strain (Uaf1 sKO), and found that Uaf1 is essential for spermiogenesis and male fertility. We found that UAF1 interacts and colocalizes with USP1 in testes. Conditional knockout of Uaf1 in testes results in disturbed expression and localization of USP1, suggesting that UAF1 regulates spermiogenesis through the function of the deubiquitinating enzyme USP1. We used tandem mass tag-based proteomics to identify differentially expressed proteins and potential underlying mechanisms, and found that conditional knockout Uaf1 in testes results in reduced levels of proteins essential for spermiogenesis. Thus, the UAF1/USP1 deubiquitinase complex is essential for normal spermiogenesis by regulating the levels of spermiogenesis-related proteins.
HostingRepository	PRIDE
AnnounceDate	2024-02-07
AnnouncementXML	Submission_2024-02-07_00:15:00.159.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziqi Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-13 06:19:45	ID requested
⏵ 1	2024-02-07 00:15:00	announced

Dataset with its publication pending

submitter keyword: UAF1;USP1;male infertility;spermiogenesis;deubiquitylation

Ziqi Wang
contact affiliation	Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China,
contact email	wangziqi1110@126.com
lab head
Ziqi Wang
contact affiliation	Shandong University
contact email	wangziqi1110@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046120
     1. Label: PRIDE project
     2. Name: The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis