PXD046108 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomic analysis identifies specific signature proteins in small extracellular vesicles from granulocytes and CD4+ T cell subpopulations |
Description | Proteomic analysis of small extracellular vesicles (sEV) is a powerful tool for the characterization of cell-to-cell signalling. While several studies describe the proteome profile of different immune cell types, none have analysed the content of their delivered sEV. The aim of the present study was to compare the protein signature of sEV delivered from cell subpopulations with importance for chronic inflammatory diseases: granulocytes (i.e., neutrophils and eosinophils) and CD4+ T cells (i.e., TH1, TH2 and TH17). Qualitative (DDA) and quantitative (DIA-SWATH) proteomic analysis of in vitro produced sEV resulted in the identification of proteomic differences depending on the originating cell source. The main differences were found between granulocytes and TH cell-derived sEV, with higher abundance of antimicrobial proteins (e.g., LCN2, LTF, MPO) in sEV from granulocytes and an enrichment of ribosomal proteins (RPL and RPS proteins) in the TH ones. Additionally, we found differentially abundant proteins between neutrophil and eosinophil sEV (e.g., ILF2, LTF, LCN2) and among TH subsets (e.g., ISG15, ITGA4, ITGB2 or NAMPT). These results highlight the potential of sEV proteins as a source of biomarkers for diseases where the different immune cell subsets studied participate, particularly chronic inflammatory pathologies such as asthma or COPD. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-18 |
AnnouncementXML | Submission_2025-02-17_23:31:28.905.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Susana Bravo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-13 03:34:24 | ID requested | |
⏵ 1 | 2025-02-17 23:31:29 | announced | |
Publication List
Keyword List
submitter keyword: TH17, exosomes, TH2, TH1, proteomics, eosinophils,sEV, neutrophils |
Contact List
Susana Belén Bravo López |
contact affiliation | Instituto de Investigaciones Sanitarias de Santiago de Compostela |
contact email | sbbravo@gmail.com |
lab head | |
Susana Bravo |
contact affiliation | FIDIS |
contact email | sbbravo@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046108
- Label: PRIDE project
- Name: Comparative proteomic analysis identifies specific signature proteins in small extracellular vesicles from granulocytes and CD4+ T cell subpopulations