PXD046108

PXD046108 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis identifies specific signature proteins in small extracellular vesicles from granulocytes and CD4+ T cell subpopulations
Description	Proteomic analysis of small extracellular vesicles (sEV) is a powerful tool for the characterization of cell-to-cell signalling. While several studies describe the proteome profile of different immune cell types, none have analysed the content of their delivered sEV. The aim of the present study was to compare the protein signature of sEV delivered from cell subpopulations with importance for chronic inflammatory diseases: granulocytes (i.e., neutrophils and eosinophils) and CD4+ T cells (i.e., TH1, TH2 and TH17). Qualitative (DDA) and quantitative (DIA-SWATH) proteomic analysis of in vitro produced sEV resulted in the identification of proteomic differences depending on the originating cell source. The main differences were found between granulocytes and TH cell-derived sEV, with higher abundance of antimicrobial proteins (e.g., LCN2, LTF, MPO) in sEV from granulocytes and an enrichment of ribosomal proteins (RPL and RPS proteins) in the TH ones. Additionally, we found differentially abundant proteins between neutrophil and eosinophil sEV (e.g., ILF2, LTF, LCN2) and among TH subsets (e.g., ISG15, ITGA4, ITGB2 or NAMPT). These results highlight the potential of sEV proteins as a source of biomarkers for diseases where the different immune cell subsets studied participate, particularly chronic inflammatory pathologies such as asthma or COPD.
HostingRepository	PRIDE
AnnounceDate	2025-02-18
AnnouncementXML	Submission_2025-02-17_23:31:28.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-13 03:34:24	ID requested
⏵ 1	2025-02-17 23:31:29	announced

10.3390/IJMS251910848;

submitter keyword: TH17, exosomes, TH2, TH1, proteomics, eosinophils,sEV, neutrophils

Susana Belén Bravo López
contact affiliation	Instituto de Investigaciones Sanitarias de Santiago de Compostela
contact email	sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD046108

PRIDE project URI

• PRIDE
  1. PXD046108
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis identifies specific signature proteins in small extracellular vesicles from granulocytes and CD4+ T cell subpopulations