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PXD046108

PXD046108 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomic analysis identifies specific signature proteins in small extracellular vesicles from granulocytes and CD4+ T cell subpopulations
DescriptionProteomic analysis of small extracellular vesicles (sEV) is a powerful tool for the characterization of cell-to-cell signalling. While several studies describe the proteome profile of different immune cell types, none have analysed the content of their delivered sEV. The aim of the present study was to compare the protein signature of sEV delivered from cell subpopulations with importance for chronic inflammatory diseases: granulocytes (i.e., neutrophils and eosinophils) and CD4+ T cells (i.e., TH1, TH2 and TH17). Qualitative (DDA) and quantitative (DIA-SWATH) proteomic analysis of in vitro produced sEV resulted in the identification of proteomic differences depending on the originating cell source. The main differences were found between granulocytes and TH cell-derived sEV, with higher abundance of antimicrobial proteins (e.g., LCN2, LTF, MPO) in sEV from granulocytes and an enrichment of ribosomal proteins (RPL and RPS proteins) in the TH ones. Additionally, we found differentially abundant proteins between neutrophil and eosinophil sEV (e.g., ILF2, LTF, LCN2) and among TH subsets (e.g., ISG15, ITGA4, ITGB2 or NAMPT). These results highlight the potential of sEV proteins as a source of biomarkers for diseases where the different immune cell subsets studied participate, particularly chronic inflammatory pathologies such as asthma or COPD.
HostingRepositoryPRIDE
AnnounceDate2025-02-18
AnnouncementXMLSubmission_2025-02-17_23:31:28.905.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusana Bravo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-13 03:34:24ID requested
12025-02-17 23:31:29announced
Publication List
10.3390/IJMS251910848;
Keyword List
submitter keyword: TH17, exosomes, TH2, TH1, proteomics, eosinophils,sEV, neutrophils
Contact List
Susana Belén Bravo López
contact affiliationInstituto de Investigaciones Sanitarias de Santiago de Compostela
contact emailsbbravo@gmail.com
lab head
Susana Bravo
contact affiliationFIDIS
contact emailsbbravo@gmail.com
dataset submitter
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Dataset FTP location
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