PXD046103

PXD046103 is an original dataset announced via ProteomeXchange.

Title	Protein and cytokine profiles associated with COVID-19 disease severity in Puerto Rico
Description	Viral strains, age, and host factors including genetics and proteins are associated with variable immune responses against SARS-CoV-2 and disease severity. We hypothesized that unique proteins/pathways are associated with COVID-19 disease severity in Puerto Rican Hispanics. A total of 121 men and women aged 21-80 years-old were recruited in Puerto Rico. Plasma samples were collected from unvaccinated COVID-19 infected subjects during acute disease (n=39) and compared to COVID-19 negative individuals (n=56) during acute disease using proteomics and cytokine expression. Infected individuals were stratified based on symptomatology as follows: mild (n=18), moderate (n=13), and severe (n=8). Quantitative proteomics was performed in plasma samples using Tandem Mass Tag (TMT) labeling. Cytokines in plasma were quantified using a human cytokine array. Proteomics analyses revealed 56 differentially regulated proteins and the top 3 pathways that were predicted to be inhibited in severe patients including LXR/RXR signaling, Production of NO and ROS in macrophages, and Synaptogenesis signaling. Decreased cadherin-13 validated by ELISA, which participates in synaptogenesis, is a novel protein is a novel protein not previously reported in other studies of COVID-19 severity and validated by ELISA. Cytokine analyses showed that TNF⍺ levels decreased with disease severity. This study uncovers potential host predictors of COVID-19 severity and new avenues for treatment in Puerto Rican Hispanics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:45:22.550.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046103
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Loyda Melendez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-12 21:56:29	ID requested
1	2024-06-15 23:46:59	announced
⏵ 2	2024-10-22 06:45:24	announced	2024-10-22: Updated project metadata.

10.6019/PXD046103;

Rosario-Rodr, í, guez LJ, Cantres-Rosario YM, Carrasquillo-Carri, ó, n K, Rosa-D, í, az A, Rodr, í, guez-De Jes, ú, s AE, Rivera-Nieves V, Tosado-Rodr, í, guez EL, M, é, ndez LB, Roche-Lima A, Bertr, á, n J, Mel, é, ndez LM, Plasma Proteins Associated with COVID-19 Severity in Puerto Rico. Int J Mol Sci, 25(10):(2024) [pubmed]

10.3390/ijms25105426;

submitter keyword: COVID-19, proteomics, plasma, SARS-CoV-2, Cahderin-13, Puerto Rico

Loyda Melendez
contact affiliation	University of Puerto Rico, Medical Sciences Campus
contact email	lmelendezlab@gmail.com
lab head
Loyda Melendez
contact affiliation	University of Puerto Rico Medical Sciences Campus
contact email	lmelendezlab@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046103
     1. Label: PRIDE project
     2. Name: Protein and cytokine profiles associated with COVID-19 disease severity in Puerto Rico