PXD046101

PXD046101 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics reveals the protective effects of Yinchenzhufu decoction against cholestatic liver fibrosis in mice by inhibiting PDGFRβ/PI3K/AKT pathway
Description	Yinchenzhufu decoction (YCZFD) is a traditional Chinese medicine formula with hepatoprotective effects. In this study, the protective effects of YCZFD against cholestatic liver fibrosis and its mechanisms of action were evaluated in a mouse model of 3,5-diethoxycarbonyl-1,4-dihydro-collidine (DDC)-induced cholestasis. YCZFD significantly decreased levels of serum biochemical, liver injury, and fibrosis indicators. Data-independent acquisition-based mass spectrometry (MS) was used to investigate proteomic changes in the livers of mice in three groups: control, model, and model treated by high-dose YCZFD. In total, 460 differentially expressed proteins (DEPs) were identified. Enrichment analyses of these DEPs uncovered that YCZFD influences multiple pathways, including PI3K-Akt, focal adhesion, ECM-receptor interaction, glutathione metabolism, and steroid biosynthesis pathways. PDGFRβ, a receptor associated with the PI3K/AKT and focal adhesion pathways, was significantly upregulated in the livers of cholestatic mice but downregulated by YCZFD. The effects of YCZFD on the expression levels of key proteins in the PDGFRβ/PI3K/AKT pathway were further confirmed in TGFβ-induced hepatic stellate cells. Finally, we uncovered six components (i.e., chlorogenic acid, scoparone, isoliquiritigenin, glycyrrhetinic acid, formononetin, atractylenolide I, and benzoylaconitine) of YCZFD that may regulate PDGFRβ expression. Overall, YCZFD exerts a substantial protective effect against DDC-induced cholestatic liver fibrosis mainly through regulating the PDGFRβ/PI3K/AKT signaling pathway.
HostingRepository	iProX
AnnounceDate	2023-10-13
AnnouncementXML	Submission_2023-10-12_20:17:00.165.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Meng
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-12 20:16:48	ID requested
⏵ 1	2023-10-12 20:17:00	announced

Dataset with its publication pending

submitter keyword: Yinchenzhufu decoction, proteomics, cholestasis, liver fibrosis, PDGFRβ/PI3K/AKT

Yueming Ma
contact affiliation	Shanghai University of Traditional Chinese Medicine
contact email	mayueming_117@hotmail.com
lab head
Qian Meng
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	mqfly0623@126.com
dataset submitter

iProX dataset URI