PXD046098

PXD046098 is an original dataset announced via ProteomeXchange.

Title	Structural and Metabolic Transitions of C4 Leaf Development and Differentiation Defined by Microscopy and Quantitative Proteomics in Maize
Description	C4 grasses, such as maize (Zea mays), have high photosynthetic efficiency through combined biochemical and structural adaptations.C4 photosynthesis is established along the developmental axis of the leafblade, leading from an undifferentiated leaf base just above the ligule into highly specialized mesophyll cells (MCs) and bundle sheath cells (BSCs) at the tip. To resolve the kinetics of maize leaf development and C4 differentiation and to obtain a systems-level understanding of maize leaf formation, the accumulation profiles of proteomes of the leaf and the isolated BSCs with their vascular bundle along the developmental gradient were determined using large-scale mass spectrometry. This was complemented by extensive qualitative and quantitative microscopy analysis of structural features (e.g., Kranz anatomy, plasmodesmata, cell wall, and organelles). More than 4300 proteins were identified and functionally annotated. Developmental protein accumulation profiles and hierarchical cluster analysis then determined the kinetics of organelle biogenesis, formation of cellular structures, metabolism, and coexpression patterns. Two main expression clusters were observed, each divided in subclusters, suggesting that a limited number of developmental regulatory networks organize concerted protein accumulation along the leaf gradient. The coexpression with BSC and MC markers provided strong candidates for further analysis of C4 specialization, in particular transporters and biogenesis factors. Based on the integrated information, we describe five developmental transitions that provide a conceptual and practical template for further analysis. An online protein expression viewer is provided through the PlantProteomeDatabase.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:56.847.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046098
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tami Leppert
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-12 15:04:02	ID requested
1	2023-10-12 15:58:33	announced
⏵ 2	2023-11-14 09:05:57	announced	2023-11-14: Updated project metadata.

Majeran W, Friso G, Ponnala L, Connolly B, Huang M, Reidel E, Zhang C, Asakura Y, Bhuiyan NH, Sun Q, Turgeon R, van Wijk KJ, Structural and metabolic transitions of C4 leaf development and differentiation defined by microscopy and quantitative proteomics in maize. Plant Cell, 22(11):3509-42(2010) [pubmed]

10.6019/PXD046098;

submitter keyword: Microscopy, developmental gradient, bundle sheet,Maize, C4 differentiation, Proteomics

Klaas van Wijk
contact affiliation	School of Integrative Plant Science Plant Biology Section, Cornell University
contact email	kv35@cornell.edu
lab head
Tami Leppert
contact affiliation	Institute for Systems Biology
contact email	tleppert@systemsbiology.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046098
     1. Label: PRIDE project
     2. Name: Structural and Metabolic Transitions of C4 Leaf Development and Differentiation Defined by Microscopy and Quantitative Proteomics in Maize