PXD046096

PXD046096 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis of the bladder and its sensitivity to inosine in a rat model of spinal cord injury
Description	This study investigates the molecular changes in the bladder following spinal cord injury (SCI) in rats and the impact of inosine treatment using a multi-omics approach. We discovered the activation of PARP (Poly(ADP-ribose) polymerase) in response to SCI, a previously unrecognized phenomenon, and its attenuation with inosine treatment. SCI triggered significant DNA damage and oxidative stress pathways, whereas inosine treatment prevents DNA damage and inhibits PARP activation, offering a potential therapeutic avenue. The integrated analysis of transcriptomics and proteomics data revealed concordant regulation of multiple pathways following SCI, including EIF2 signaling and NRF2-mediated oxidative stress response, which are ameliorated by inosine treatment. These findings have relevance to human neurogenic bladder pathobiology. Pathway inhibition by inosine in the setting of SCI suggests its potential for neuroprotection in the bladder. Despite limitations, such as the focus on male rats and a lack of proteomics data from separated detrusor and mucosa, this study provides valuable insights into the molecular mechanisms underlying bladder dysfunction following SCI. It also suggests the repurposing of FDA-approved PARP inhibitors for the treatment of bladder dysfunction following spinal injury.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:09:50.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Yang
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-12 13:39:59	ID requested
⏵ 1	2025-07-13 16:09:51	announced

10.1172/jci.insight.180275;

Hashemi Gheinani A, Sack BS, Bigger-Allen A, Thaker H, Atta H, Lambrinos G, Costa K, Doyle C, Gharaee-Kermani M, Patalano S, Piper M, Cotellessa JF, Vitko D, Li H, Kadayil Prabhakaran M, Cristofaro V, Froehlich J, Lee RS, Yang W, Sullivan MP, Macoska JA, Adam RM, Multiomics analysis unveils an inosine-sensitive DNA damage response in neurogenic bladder after spinal cord injury. JCI Insight, 10(12):(2025) [pubmed]

submitter keyword: Multi-Omics, Spinal Cord Injury, Inosine, Bladder, Rat

Wei Yang
contact affiliation	Department of Pathology Cancer Center Stony Brook University
contact email	wei.yang@stonybrook.edu
lab head
Wei Yang
contact affiliation	Stony Brook University
contact email	omician@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046096
     1. Label: PRIDE project
     2. Name: Multi-omics analysis of the bladder and its sensitivity to inosine in a rat model of spinal cord injury