PXD046086

PXD046086 is an original dataset announced via ProteomeXchange.

Title	The antimicrobial peptide Esc(1-21)-1c increases susceptibility of Pseudomonas aeruginosa to conventional antibiotics by decreasing the expression of the MexAB-OprM efflux pump
Description	The increase in bacterial strains resistant to conventional antibiotics is an alarming problem for human health and could lead to pandemics in the future. Among bacterial pathogens responsible for a large variety of severe infections there is Pseudomonas aeruginosa. Therefore, there is an urgent need for new molecules with antimicrobial activity or that can act as adjuvants of antibiotics already in use. In this scenario, antimicrobial peptides (AMPs) hold great promise. Recently, we characterized a frogskin AMP derived from esculentin-1a, namely Esc(1-21)-1c, endowed with antipseudomonal activity without being cytotoxic to human cells. Here, we investigated the effect of combining this peptide with a panel of antibiotics from different classes and found that Esc(1-21)-1c can synergistically inhibit the growth of P. aeruginosa cells with three different antibiotics, including tetracycline. We therefore investigated the underlying mechanism implemented by the peptide using a differential proteomic approach. The data revealed a significant decrease in the production of three proteins belonging to the MexAB-OprM efflux pump upon treatment with sub-inhibitory concentration of Esc(1-21)-1c. Downregulation of these proteins was confirmed by transcriptional analysis and direct measurement of their relative levels in bacterial cells by tandem mass spectrometry analysis in multiple reaction monitoring scan mode. This would increase the intracellular drug content making bacteria more susceptible to the antibiotic. Overall, these results highlight the importance of characterizing new molecules able to synergize with conventional antibiotics, paving the way for the development of alternative therapeutic strategies based on AMP/antibiotic formulations to counteract the emergence of resistant bacterial strains and increase the use of "old" antibiotics in medical practice.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:06:18.273.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	angela di somma
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-12 03:40:08	ID requested
⏵ 1	2024-01-26 06:06:19	announced

10.3389/fchem.2023.1271153;

Can, è C, Casciaro B, Di Somma A, Loffredo MR, Puglisi E, Battaglia G, Mellini M, Cappiello F, Rampioni G, Leoni L, Amoresano A, Duilio A, Mangoni ML, to conventional antibiotics by decreasing the expression of the MexAB-OprM efflux pump. Front Chem, 11():1271153(2023) [pubmed]

submitter keyword: antibiotics, Antimicrobial peptide,Pseudomonas aeruginosa, proteomic, efflux pumps, Tetracycline

Angela Duilio
contact affiliation	Department of Chemical Sciences, University of Naples “Federico II”, Naples, Italy.
contact email	angela.duilio@unina.it
lab head
angela di somma
contact affiliation	Federico Secondo ii
contact email	angela.disomma@unina.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046086
     1. Label: PRIDE project
     2. Name: The antimicrobial peptide Esc(1-21)-1c increases susceptibility of Pseudomonas aeruginosa to conventional antibiotics by decreasing the expression of the MexAB-OprM efflux pump