PXD046085

PXD046085 is an original dataset announced via ProteomeXchange.

Title	The characterisation of protein and cellular binding in paediatric extracorporeal membrane oxygenation (ECMO) circuits using proteomics, immunofluorescence, and scanning electron microscopy
Description	Extracorporeal membrane oxygenation (ECMO) has been increasingly applied to both adult and paediatric patients worldwide. However, continuous contact between blood and foreign surface of the ECMO circuit may contribute to haemostatic, inflammatory and other physiological disturbances during ECMO. Although previous studies have extensively investigated blood samples from patients on ECMO, the protein and cellular binding to the ECMO circuit has largely been overlooked as an additional factor that could contribute to differences in clinical outcomes. The detailed protocol described in this paper enables the comprehensive characterisation of protein and cellular binding in paediatric ECMO circuits, which could extend our knowledge of the pathophysiology of circuit binding and provide guidance for improved ECMO circuit design.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_04:03:53.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-12 03:35:46	ID requested
⏵ 1	2024-10-07 04:03:54	announced

10.1097/MAT.0000000000002273;

submitter keyword: ECMO, proteomics

Prof Vera Ignjatovic
contact affiliation	Prof Vera Ignjatovic, Professor (PAR) of Pediatrics Head, Translational Research and Innovation Laboratory (TRaIL), Johns Hopkins All Children's Institute for Clinical and Translational Research
contact email	vignjat1@jhmi.edu
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD046085

PRIDE project URI

• PRIDE
  1. PXD046085
     1. Label: PRIDE project
     2. Name: The characterisation of protein and cellular binding in paediatric extracorporeal membrane oxygenation (ECMO) circuits using proteomics, immunofluorescence, and scanning electron microscopy