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PXD046076

PXD046076 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlmS plays a key role in the virulence and biofilm formation ability of Staphylococcus aureus promoted by advanced glycation end products
DescriptionStaphylococcus aureus (S. aureus) is well known for its biofilm formation ability and is responsible for serious, chronic refractory infections worldwide. We previously demonstrated that advanced glycation end products (AGEs), a hallmark of chronic hyperglycemia in diabetic tissues, enhanced biofilm formation by promoting eDNA release via sigB upregulation in S. aureus, contributing to the high morbidity and mortality of diabetic foot ulcer infection. However, the exact regulatory network has not been completely described. Here, we used a pull-down assay and LC‒MS/MS to identify the GlmS protein as a candidate regulator of sigB in S. aureus stimulated by AGEs. Dual-luciferase assays and electrophoretic mobility shift assays (EMSAs) revealed that GlmS directly upregulated the transcriptional activity of sigB. We constructed Newman ∆glmS for further validation. Quantitative RT‒PCR analysis revealed that AGEs promoted both glmS and sigB expression in the Newman strain but had no effect on Newman ∆glmS. Newman ∆glmS showed a significant attenuation in biofilm formation ability and virulence, accompanied by a decrease in sigB expression, even under AGE stimulation. All of the changes, including pigment deficiency, decreased hemolysis ability, downregulation of hla and hld expression, and less and sparser biofilms, indicated that sigB and biofilm formation ability no longer responded to AGEs in Newman ∆glmS. Our data extend the understanding of GlmS in the global regulatory network of S. aureus and demonstrate a new mechanism by which AGEs can upregulate GlmS, which subsequently directly regulates sigB and plays a significant role in mediating biofilm formation and virulence factor expression.
HostingRepositoryPRIDE
AnnounceDate2024-05-22
AnnouncementXMLSubmission_2024-05-22_02:30:28.652.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRUI SHEN
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-11 20:34:09ID requested
12024-05-22 02:30:29announced
Publication List
Ni L, Shen R, Luo H, Li X, Zhang X, Huang L, Deng Y, Liao X, Wu Y, Duan C, Xie X, promoted by advanced glycation end products. Virulence, 15(1):2352476(2024) [pubmed]
10.1080/21505594.2024.2352476;
Keyword List
submitter keyword: Staphylococcus aureus
advanced glycation end products
biofilm formation
GlmS-sigB regulatory axis
Contact List
Xiaoying Xie
contact affiliationSun Yat-sen Memorial Hospital,Sun Yat-sen University 107 Yanjiang West Road Guangzhou 510120, P. R. China
contact emailxiexy5@mail.sysu.edu.cn
lab head
RUI SHEN
contact affiliationSun Yat-sen Memorial Hospital
contact emailsr_three@163.com
dataset submitter
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Dataset FTP location
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