PXD046075

PXD046075 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of Chrysoporthe cubensis exoproteomes and their specificity for saccharification of sugarcane bagasse
Description	The phytopathogenic fungus Chrysoporthe cubensis is a relevant source of lignocellulolytic enzymes. This work aimed to compare the profile of lignocellulose- degrading proteins secreted by C. cubensis grown under semi-solid state fermentation using wheat bran and sugarcane bagasse. The proteins from the fungus extract grown in wheat bran (WBE) and sugarcane bagasse (SBE) were qualitative and quantitatively analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS). Label-free proteomic analysis of WBE and SBE showed that the fungus produced a spectrum of carbohydrate-active enzymes (CAZymes) with exclusive characteristics from each extract. While SBE resulted in an enzymatic profile directed towards the depolymerization of cellulose, the enzymes in WBE were more adaptable to the degradation of biomass rich in hemicellulose and other non-lignocellulosic polymers. Saccharification of alkaline pre-treated sugarcane bagasse with SBE promoted glucose release higher than commercial cocktails (8.11 g L -1 ), while WBE promoted the higher release of xylose (5.71 g L -1 ). Our results allowed an in-depth knowledge of the complex set of enzymes secreted by C. cubensis responsible for its high lignocellulolytic activity and still provided the identification of promising target proteins for biotechnological applications in the context of biorefinery.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:42:46.812.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	William Borges
SpeciesList	scientific name: Chrysoporthe cubensis; NCBI TaxID: 305400; scientific name: Fungi; NCBI TaxID: NCBITaxon:4751;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-11 19:18:20	ID requested
⏵ 1	2024-01-26 06:42:47	announced

10.1016/j.enzmictec.2023.110365;

Tavares MP, Morgan T, Gomes RF, Mendes JPR, Castro-Borges W, Maitan-Alfenas GP, Guimar, ã, es VM, Comparative analysis of Chrysoporthe cubensis exoproteomes and their specificity for saccharification of sugarcane bagasse. Enzyme Microb Technol, 173():110365(2024) [pubmed]

submitter keyword: Chrysoporthe cubensis

wheat bran

sugarcane bagasse

CAZymes

exoproteome

biomass saccharification

William Castro-Borges
contact affiliation	Department of Biological Sciences, Federal University of Ouro Preto, Minas Gerais, Brazil.
contact email	wborges@ufop.edu.br
lab head
William Borges
contact affiliation	Universidade Federal de Ouro Preto
contact email	wborges@ufop.edu.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046075
     1. Label: PRIDE project
     2. Name: Comparative analysis of Chrysoporthe cubensis exoproteomes and their specificity for saccharification of sugarcane bagasse