PXD046070
PXD046070 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A rapid, tag-free way to purify functional GPCRs |
Description | A GPCR purification method is described based on how the Galpha-CT (alpha5 helix) interacts with G-protein coupled receptors (GPCRs). The importance of this region in binding to and stabilizing the active GPCR state (R*) is well established, as it plays a conserved role in allosterically linking R* binding to Gα activation. Although only about 20 amino acids, the Galpha-CT contributes approximately 50% of the total interacting surface area with active receptors (R*). Galpha-CT binding also activates the G protein, by triggering conformational changes in the Galpha subunit that facilitate GDP – GTP exchange and heterotrimer dissociation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-22 |
AnnouncementXML | Submission_2024-05-22_02:29:33.235.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Phillip Wilmarth |
SpeciesList | scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-11 11:39:45 | ID requested | |
⏵ 1 | 2024-05-22 02:29:34 | announced |
Publication List
Shumate AD, Farrens DL, A rapid, tag-free way to purify functional GPCRs. J Biol Chem, 300(1):105558(2024) [pubmed] |
10.1016/j.jbc.2023.105558; |
Keyword List
submitter keyword: in-gel digestion, affinity purification, mass spectrometry,GPCP purification, protein identification |
Contact List
David Farrens, Ph.D. | |
---|---|
contact affiliation | Chemical Physiology and Biochemistry Oregon Health and Science University Portland, OR 97239 USA |
contact email | farrensd@ohsu.edu |
lab head | |
Phillip Wilmarth | |
contact affiliation | OHSU |
contact email | wilmarth@ohsu.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD046070 |
PRIDE project URI |
Repository Record List
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