PXD046070

PXD046070 is an original dataset announced via ProteomeXchange.

Title	A rapid, tag-free way to purify functional GPCRs
Description	A GPCR purification method is described based on how the Galpha-CT (alpha5 helix) interacts with G-protein coupled receptors (GPCRs). The importance of this region in binding to and stabilizing the active GPCR state (R*) is well established, as it plays a conserved role in allosterically linking R* binding to Gα activation. Although only about 20 amino acids, the Galpha-CT contributes approximately 50% of the total interacting surface area with active receptors (R*). Galpha-CT binding also activates the G protein, by triggering conformational changes in the Galpha subunit that facilitate GDP – GTP exchange and heterotrimer dissociation.
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_02:29:33.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-11 11:39:45	ID requested
⏵ 1	2024-05-22 02:29:34	announced

Shumate AD, Farrens DL, A rapid, tag-free way to purify functional GPCRs. J Biol Chem, 300(1):105558(2024) [pubmed]

10.1016/j.jbc.2023.105558;

submitter keyword: in-gel digestion, affinity purification, mass spectrometry,GPCP purification, protein identification

David Farrens, Ph.D.
contact affiliation	Chemical Physiology and Biochemistry Oregon Health and Science University Portland, OR 97239 USA
contact email	farrensd@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD046070

PRIDE project URI

• PRIDE
  1. PXD046070
     1. Label: PRIDE project
     2. Name: A rapid, tag-free way to purify functional GPCRs