PXD046068

PXD046068 is an original dataset announced via ProteomeXchange.

Title	The RNA helicase Dbp10 coordinates assembly factor association with PTC formation during ribosome biogenesis
Description	During ribosome biogenesis a plethora of assembly factors and essential enzymes drive the unidirectional maturation of nascent pre-ribosomal subunits. The DEAD-box RNA helicase Dbp10 is suggested to restructure pre-ribosomal rRNA of the evolving peptidyl-transferase center (PTC) on nucleolar ribosomal 60S assembly intermediates. Here, we show that point mutations within conserved catalytic helicase-core motifs of Dbp10 yield a dominant-lethal growth phenotype. Such dbp10 mutants, which stably associate with pre-60S intermediates, impair pre-60S biogenesis at a nucleolar stage prior to the release of assembly factor Rrp14 and stable integration of late nucleolar factors such as Noc3. Furthermore, the binding of the GTPase Nug1 to particles isolated directly via mutant Dbp10 bait proteins is specifically inhibited. The N-terminal domain of Nug1 interacts with Dbp10 and the methyltransferase Spb1, whose pre-60S incorporation is also reduced in absence of functional Dbp10. Our data suggest that Dbp10’s helicase activity generates a crucial framework for assembly factor docking thereby permitting the progression of pre-60S maturation
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_05:34:41.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalia Kunowska
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-11 10:05:47	ID requested
⏵ 1	2024-05-22 05:34:42	announced

10.1093/nar/gkad1206;

Mitterer V, Hamze H, Kunowska N, Stelzl U, Henras AK, Hurt E, The RNA helicase Dbp10 coordinates assembly factor association with PTC maturation during ribosome biogenesis. Nucleic Acids Res, 52(4):1975-1987(2024) [pubmed]

submitter keyword: ribosome assembly. pre-60S, assembly factors, S. cerevisiae

Ulrich Stelzl
contact affiliation	University of Graz and BioTechMed-Graz Institute of Pharmaceutical Sciences Pharmaceutical Chemistry Universitätsplatz 1/I A-8010 Graz, Austria phone: +43(0)316380-5397
contact email	ulrich.stelzl@uni-graz.at
lab head
Natalia Kunowska
contact affiliation	Institute of Pharmaceutical Sciences University of Graz
contact email	natalia.kunowska@uni-graz.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046068
     1. Label: PRIDE project
     2. Name: The RNA helicase Dbp10 coordinates assembly factor association with PTC formation during ribosome biogenesis