PXD046068 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The RNA helicase Dbp10 coordinates assembly factor association with PTC formation during ribosome biogenesis |
Description | During ribosome biogenesis a plethora of assembly factors and essential enzymes drive the unidirectional maturation of nascent pre-ribosomal subunits. The DEAD-box RNA helicase Dbp10 is suggested to restructure pre-ribosomal rRNA of the evolving peptidyl-transferase center (PTC) on nucleolar ribosomal 60S assembly intermediates. Here, we show that point mutations within conserved catalytic helicase-core motifs of Dbp10 yield a dominant-lethal growth phenotype. Such dbp10 mutants, which stably associate with pre-60S intermediates, impair pre-60S biogenesis at a nucleolar stage prior to the release of assembly factor Rrp14 and stable integration of late nucleolar factors such as Noc3. Furthermore, the binding of the GTPase Nug1 to particles isolated directly via mutant Dbp10 bait proteins is specifically inhibited. The N-terminal domain of Nug1 interacts with Dbp10 and the methyltransferase Spb1, whose pre-60S incorporation is also reduced in absence of functional Dbp10. Our data suggest that Dbp10’s helicase activity generates a crucial framework for assembly factor docking thereby permitting the progression of pre-60S maturation |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-22 |
AnnouncementXML | Submission_2024-05-22_05:34:41.281.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Natalia Kunowska |
SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | acetylated residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-11 10:05:47 | ID requested | |
⏵ 1 | 2024-05-22 05:34:42 | announced | |
Publication List
10.1093/nar/gkad1206; |
Mitterer V, Hamze H, Kunowska N, Stelzl U, Henras AK, Hurt E, The RNA helicase Dbp10 coordinates assembly factor association with PTC maturation during ribosome biogenesis. Nucleic Acids Res, 52(4):1975-1987(2024) [pubmed] |
Keyword List
submitter keyword: ribosome assembly. pre-60S, assembly factors, S. cerevisiae |
Contact List
Ulrich Stelzl |
contact affiliation | University of Graz and BioTechMed-Graz Institute of Pharmaceutical Sciences Pharmaceutical Chemistry Universitätsplatz 1/I A-8010 Graz, Austria phone: +43(0)316380-5397 |
contact email | ulrich.stelzl@uni-graz.at |
lab head | |
Natalia Kunowska |
contact affiliation | Institute of Pharmaceutical Sciences University of Graz |
contact email | natalia.kunowska@uni-graz.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046068
- Label: PRIDE project
- Name: The RNA helicase Dbp10 coordinates assembly factor association with PTC formation during ribosome biogenesis