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PXD046068

PXD046068 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe RNA helicase Dbp10 coordinates assembly factor association with PTC formation during ribosome biogenesis
DescriptionDuring ribosome biogenesis a plethora of assembly factors and essential enzymes drive the unidirectional maturation of nascent pre-ribosomal subunits. The DEAD-box RNA helicase Dbp10 is suggested to restructure pre-ribosomal rRNA of the evolving peptidyl-transferase center (PTC) on nucleolar ribosomal 60S assembly intermediates. Here, we show that point mutations within conserved catalytic helicase-core motifs of Dbp10 yield a dominant-lethal growth phenotype. Such dbp10 mutants, which stably associate with pre-60S intermediates, impair pre-60S biogenesis at a nucleolar stage prior to the release of assembly factor Rrp14 and stable integration of late nucleolar factors such as Noc3. Furthermore, the binding of the GTPase Nug1 to particles isolated directly via mutant Dbp10 bait proteins is specifically inhibited. The N-terminal domain of Nug1 interacts with Dbp10 and the methyltransferase Spb1, whose pre-60S incorporation is also reduced in absence of functional Dbp10. Our data suggest that Dbp10’s helicase activity generates a crucial framework for assembly factor docking thereby permitting the progression of pre-60S maturation
HostingRepositoryPRIDE
AnnounceDate2024-05-22
AnnouncementXMLSubmission_2024-05-22_05:34:41.281.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNatalia Kunowska
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListacetylated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-11 10:05:47ID requested
12024-05-22 05:34:42announced
Publication List
10.1093/nar/gkad1206;
Mitterer V, Hamze H, Kunowska N, Stelzl U, Henras AK, Hurt E, The RNA helicase Dbp10 coordinates assembly factor association with PTC maturation during ribosome biogenesis. Nucleic Acids Res, 52(4):1975-1987(2024) [pubmed]
Keyword List
submitter keyword: ribosome assembly. pre-60S, assembly factors, S. cerevisiae
Contact List
Ulrich Stelzl
contact affiliationUniversity of Graz and BioTechMed-Graz Institute of Pharmaceutical Sciences Pharmaceutical Chemistry Universitätsplatz 1/I A-8010 Graz, Austria phone: +43(0)316380-5397
contact emailulrich.stelzl@uni-graz.at
lab head
Natalia Kunowska
contact affiliationInstitute of Pharmaceutical Sciences University of Graz
contact emailnatalia.kunowska@uni-graz.at
dataset submitter
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