PXD046030

PXD046030 is an original dataset announced via ProteomeXchange.

Title	Structural Similarities on Hymenoptera Allergenic Dipeptidyl Peptidases IV (DPPIVs). Overall comparison also including a new DPPIV sequence from Vespa velutina.
Description	(1)Background: Dipeptidyl Peptidases IV (DPPIVs), present in many organisms, are minor components in the venoms of Hymenoptera, where they have been shown as cross-reactive allergenic molecules. Since the structure of homologous DPPIVs is well characterized, we explain which regions have higher similarity among these proteins and present a comparison including a new Vespa velutina DPPIV sequence. Moreover, two cases of sensitization to DPPIV in wasps- and honeybees-sensitized patients are presented. (2) Methods: Proteomic analyses have been performed on the venom of the Asian Hornet V.velutina, in order to demonstrate the sequence of its DPPIV (putative allergen Vesp v 3). Comparison by alignments and analysis of the three-dimensional structure allow to show a region with higher similarity among Hymenoptera DPPIVs. Besides, ImmunoCAP™ determinations (including specific inhibition experiments), as well as IgE-immunoblotting, demonstrate the presence of Api m 5 and Ves v 3. (3) Results and conclusions: The data presented explain that the similarities among Hymenoptera DPPIVs are most probably localized at the C-terminal region of these enzymes. The clinical cases analyzed demonstrate the presence of this minor component in the preparations used in venom immunotherapy. Moreover, a new DPPIV sequence is published (Accession Number P0DRB8).
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_09:34:12.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Vespa velutina velutina; NCBI TaxID: 1881882;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-10 05:49:51	ID requested
⏵ 1	2024-01-26 09:34:13	announced

10.3390/toxins15110656;

Monsalve RI, Lombardero M, Christensen LH, N, ú, ñ, ez-Acevedo B, Gonz, á, lez-de-Olano D, Sobrino-Garc, í, a M, Castillo-Loja RM, Bravo SB, Alonso-Sampedro M, Vidal C, . Toxins (Basel), 15(11):(2023) [pubmed]

submitter keyword: DPPIV Vespa velutina allergen sequence cross-reactivity

Susana Belen Bravo Lopez
contact affiliation	Fundacion Instituto de Investigaciones Sanitarias de santiago de Compostela
contact email	sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046030
     1. Label: PRIDE project
     2. Name: Structural Similarities on Hymenoptera Allergenic Dipeptidyl Peptidases IV (DPPIVs). Overall comparison also including a new DPPIV sequence from Vespa velutina.