PXD046029

PXD046029 is an original dataset announced via ProteomeXchange.

Title	De novo identification of bacterial antigens of a clinical isolate by combining use of proteosurfaceomics, secretomics, and BacScan technologies
Description	Emerging infectious diseases pose a significant threat to both human and animal populations. Rapid de novo identification of protective antigens from a clinical isolate and development of an antigen-matched vaccine is a golden strategy to prevent the spread of emerging novel pathogens. Here, we have developed a universal pipeline by integrating proteosurfaceomics, secretomics, and BacScan technologies for the rapid de novo identification of bacterial protective proteins from a clinical isolate without prior knowledge of the pathogenic bacterium. As a proof of concept, we identified 3 novel protective proteins of A. pleuropneumoniae. Using the protective protein HBS1_14 and toxin proteins, we have developed a promising multivalent subunit vaccine against A. pleuropneumoniae. We believe that our strategy can be applied to any bacterial pathogen and has the potential to significantly accelerate the development of antigen-matched vaccines to prevent the spread of an emerging novel bacterial pathogen.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:15:32.616.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinyue Yang
SpeciesList	scientific name: Actinobacillus pleuropneumoniae; NCBI TaxID: 715;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-10 05:39:26	ID requested
1	2023-11-21 19:34:57	announced
⏵ 2	2024-10-22 06:15:32	announced	2024-10-22: Updated project metadata.

10.3389/FIMMU.2023.1274027;

submitter keyword: de novo identification, vaccine, A. pleuropneumoniae,Emerging bacterial pathogens, highly immunogenic proteins

Pan Tao
contact affiliation	Huazhong Agricultural University
contact email	taopan@mail.hzau.edu.cn
lab head
Jinyue Yang
contact affiliation	Huazhong Agricultural University
contact email	1065367325@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046029

PRIDE project URI

• PRIDE
  1. PXD046029
     1. Label: PRIDE project
     2. Name: De novo identification of bacterial antigens of a clinical isolate by combining use of proteosurfaceomics, secretomics, and BacScan technologies