PXD046012

PXD046012 is an original dataset announced via ProteomeXchange.

Title	SSSMuG: Same Sample Sequential Multi-Glycomics
Description	The mammalian glycome is structurally complex and diverse, composed of many glycan classes such as N- and O-linked glycans, glycosaminoglycans (GAGs), glycosphingolipids (GSLs) and other distinct glycan features such as polysialic acids (PolySia), sulfation and proteoglycan attachment stubs. Various methods are used to analyse these different components of the glycome, but they require pre-fractionated/partitioned samples to target each glycan class individually. To address this need for a knowledge of the relationship between the different glycan components of a biological system, we have developed a sequential release workflow for analysis of multiple conjugated glycan classes (PolySia, GAGs, GSL glycans, N-glycans O-glycans) from the same tissue lysate, termed SSSMuG: Same Sample Sequential Multi-Glycomics. With this sequential glycan release approach, five glycan classes were characterized (or four glycan classes plus proteomics) from a single sample using enzymatic or chemical release from the same spot immobilized on a polyvinylidene difluoride membrane. The various released glycan classes were then analysed by HPLC and/or MS techniques. Compared to single glycan class release approaches, SSSMuG is able to identify more glycans and more proteins with higher intensity analytical peaks and provides a better comparative normalisation of the different glycan classes of the complex glycome.
HostingRepository	PRIDE
AnnounceDate	2024-02-13
AnnouncementXML	Submission_2024-02-12_16:40:01.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Moh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-09 22:03:01	ID requested
⏵ 1	2024-02-12 16:40:02	announced

10.1021/acs.analchem.3c04928;

Moh ESX, Dalal S, DeBono NJ, Kautto L, Wongtrakul-Kish K, Packer NH, SSSMuG: Same Sample Sequential Multi-Glycomics. Anal Chem, ():(2024) [pubmed]

submitter keyword: multi-glycomics, glycome, proteomics, glycoconjugate, multi-omics,brain

Nicki Packer
contact affiliation	ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Faculty of Science and Engineering, Macquarie University, North Ryde, Sydney, NSW 2109, Australia.
contact email	nicki.packer@mq.edu.au
lab head
Edward Moh
contact affiliation	Macquarie University
contact email	edward.moh@mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046012

PRIDE project URI

• PRIDE
  1. PXD046012
     1. Label: PRIDE project
     2. Name: SSSMuG: Same Sample Sequential Multi-Glycomics