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PXD046005

PXD046005 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffect of oxygen tension on MMTV-PyMT tumor cell kinome
DescriptionSolid tumors are characterized by significantly lower oxygen (O2) levels. Despite this fact, routine preclinical cancer studies are performed under ambient O2 conditions. We have developed an experimental approach that allows us to collect, process and evaluate cancer and non-cancer cells under physioxia (3-5% O2), in such a way that there is very minimal exposure to air. We have shown in previous studies that ambient and physioxic O2 differentially impact relevant cell surface markers and sensitivity to therapy. In this study, our goal was to further explore oxygen-dependent signaling pathway alterations and to determine how these pathways influence response to targeted therapies. Using cells derived from transgenic mouse mammary tumors and healthy primary human breast epithelial cells, we show the impact of ambient air and physioxia on the kinome, with subsequent pathway alterations that mediate the effectiveness of single and combination therapies. Our data emphasize the importance of O2 considerations in preclinical cancer research and drug development.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:38:33.466.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSteven Angus
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-09 15:31:37ID requested
12024-05-08 08:33:16announced
22024-10-22 06:38:33announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ambient,breast cancer, kinase inhibitor beads, physioxia, lapatinib
Contact List
Hari Nakshatri, PhD
contact affiliationIndiana University School of Medicine
contact emailhnakshat@iupui.edu
lab head
Steven Angus
contact affiliationIndiana University School of Medicine
contact emailsangus@iu.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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