PXD045997

PXD045997 is an original dataset announced via ProteomeXchange.

Title	Lysinoalanine crosslinking is a conserved post-translational modification in the spirochete flagellar hookLysinoalanine crosslinking is a conserved post-translational modification in the spirochete flagellar hook
Description	In this project, we investigated whether diverse spirochete species contain lysinoalanine cross-linkages between conserved residues in their flagellar hook proteins (FlgE). We examined the following species: Treponema denticola¸ Borreliella burgdorferi, Treponema phagedenis, Treponema pallidum, Brachyspira hyodysenteriae, Leptospira interrogans. For each bacterial species, we examined a variety of different sample types, including recombinantly produced FlgE, polyhooks purified from flik knock-out strains, and wild-type periplasmic filaments. Overall, our data suggests that lysinoalanine crosslinking in a conserved FlgE post-translational modification in spirochetes and is required for the unique motility of these organisms. For more information, please see the following manuscript: https://doi.org/10.1101/2023.06.13.544825.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_10:02:43.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qin Fu
SpeciesList	scientific name: Borrelia sp. BC1; NCBI TaxID: 1867051;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-09 07:03:47	ID requested
⏵ 1	2024-01-26 10:02:45	announced

10.1093/pnasnexus/pgad349;

Lynch MJ, Deshpande M, Kurniyati K, Zhang K, James M, Miller M, Zhang S, Passalia FJ, Wunder EA, Charon NW, Li C, Crane BR, Lysinoalanine cross-linking is a conserved post-translational modification in the spirochete flagellar hook. PNAS Nexus, 2(12):pgad349(2023) [pubmed]

submitter keyword: post-translational modification,Spirochete, lysinoalanine

Sheng Zhang
contact affiliation	Cornell University
contact email	sz14@cornell.edu
lab head
Qin Fu
contact affiliation	Cornell University
contact email	qf44@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045997

PRIDE project URI

• PRIDE
  1. PXD045997
     1. Label: PRIDE project
     2. Name: Lysinoalanine crosslinking is a conserved post-translational modification in the spirochete flagellar hookLysinoalanine crosslinking is a conserved post-translational modification in the spirochete flagellar hook