PXD045994

PXD045994 is an original dataset announced via ProteomeXchange.

Title	The marine diterpenes pseudopteroxazole (Ptx) and pseudopterosin G (PsG) target the integrity of the gram-positive cell wall
Description	Pseudopteroxazole (Ptx) and the pseudopterosins are marine natural products with promising antibacterial potential. While Ptx has attracted interest for its anti-mycobacterial activity, pseudopterosins are active against several clinically relevant pathogens. Both compound classes exhibit low cytotoxicity and accessibility to targeted synthesis, yet their antibacterial mechanisms remain elusive. In this study, we investigated the modes of action of Ptx and pseudopterosin G (PsG) in Bacillus subtilis employing an unbiased approach that combines gel-based proteomics with a mathematical similarity analysis of response profiles. Proteomic responses to sublethal concentrations of Ptx and PsG were compared to a library of antibiotic stress response profiles revealing that both induce a stress response characteristic for agents targeting the bacterial cell envelope by interfering with membrane-bound steps of cell wall biosynthesis. Microscopy-based assays confirmed that both compounds compromise the integrity of the bacterial cell wall without disrupting the membrane potential.
HostingRepository	PRIDE
AnnounceDate	2024-01-04
AnnouncementXML	Submission_2024-01-04_06:17:16.567.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niklas Janzing
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-09 06:14:33	ID requested
⏵ 1	2024-01-04 06:17:16	announced

10.1002/PMIC.202300390;

submitter keyword: Natural Products, Pseudopteroxazole, Pseudopterosin G, CoPR

Julia Bandow
contact affiliation	Ruhr-Universität Bochum, AG Angewandte Mikrobiologie, Universitätsstraße 150, 44780 Bochum, Raum: ND 06/795, Tel.: +49 234 32 23102
contact email	julia.bandow@rub.de
lab head
Niklas Janzing
contact affiliation	Ruhr-University Bochum
contact email	niklas.janzing@rub.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045994
     1. Label: PRIDE project
     2. Name: The marine diterpenes pseudopteroxazole (Ptx) and pseudopterosin G (PsG) target the integrity of the gram-positive cell wall