PXD045971

PXD045971 is an original dataset announced via ProteomeXchange.

Title	A chromatin-regulated biphasic circuit coordinates IL1B-mediated inflammation
Description	Inflammation is characterized by a biphasic cycle consisting initially of a pro-inflammatory phase which is subsequently resolved by anti-inflammatory processes. The coordination of these two disparate states needs to be highly controlled, suggesting that the regulation of the cytokines that drive these processes are intimately linked. Interleukin-1 beta (IL1B) is a master regulator of pro-inflammation and is encoded within the same topologically associated domain (TAD) as interleukin-37 (IL37). IL37 has recently emerged as a powerful anti-inflammatory cytokine which diametrically opposes the function of IL1B. Within this TAD, we identified a novel long non-coding RNA called AMANZI which negatively regulates IL1B expression and trained immunity through the induction of IL37 transcription. We found that the activation of IL37 occurs through the formation of a dynamic long-range chromatin contact that leads to the temporal delay of anti-inflammatory responses. The common variant rs16944 present in AMANZI augments this regulatory circuit, predisposing individuals to enhanced pro-inflammation or immunosuppression. Our work illuminates a chromatin-mediated biphasic circuit coordinating expression of IL1B and IL37, thereby regulating two functionally opposed states of inflammation from within a single TAD.
HostingRepository	PRIDE
AnnounceDate	2023-10-27
AnnouncementXML	Submission_2023-10-27_10:58:30.942.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CathrinGraewe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-08 14:01:09	ID requested
⏵ 1	2023-10-27 10:58:31	announced

Dataset with its publication pending

submitter keyword: trained immunity,Topologically associated domains (TADs), long non-coding RNAs (lncRNAs), inflammation, RNA epigenetics

Musa M.Mhlanga
contact affiliation	Epigenomics & Single Cell Biophysics Group, Department of Cell Biology FNWI, Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen, 6525 HP, the Netherlands Department of Human Genetics, Radboud University Medical Center, 6525 HP, the Netherlands
contact email	ikigai@mhlangalab.org
lab head
CathrinGraewe
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands.
contact email	c.grawe@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045971

PRIDE project URI

• PRIDE
  1. PXD045971
     1. Label: PRIDE project
     2. Name: A chromatin-regulated biphasic circuit coordinates IL1B-mediated inflammation