PXD045970

PXD045970 is an original dataset announced via ProteomeXchange.

Title	Comprehensive cell-specific protein analysis in early and late pollen development from diploid microsporocytes to pollen tube growth
Description	Pollen development in angiosperms is one of the most important processes controlling plant reproduction and thus productivity. At the same time, pollen development is highly sensitive to environmental fluctuations, including temperature, drought, and nutrition. Therefore, pollen biology is a major focus in applied studies and breeding approaches for improving plant productivity in a globally changing climate. The most accessible developmental stages of pollen are the mature pollen and the pollen tubes, and these are thus most frequently analyzed. To reveal a complete quantitative proteome map, we additionally addressed the very early stages, analyzing eight stages of tobacco pollen development: diploid microsporocytes, meiosis, tetrads, microspores, polarized microspores, bipolar pollen, desiccated pollen, and pollen tubes. A protocol for the isolation of the early stages was established. Proteins were extracted and analyzed by means of a new gel LC-MS fractionation protocol. In total, 3817 protein groups were identified. Quantitative analysis was performed based on peptide count. Exceedingly stage-specific differential protein regulation was observed during the conversion from the sporophytic to the gametophytic proteome. A map of highly specialized functionality for the different stages could be revealed from the metabolic activity and pronounced differentiation of proteasomal and ribosomal protein complex composition up to protective mechanisms such as high levels of heat shock proteins in the very early stages of development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:15.584.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-08 09:21:21	ID requested
1	2023-10-08 23:08:52	announced
2	2023-11-14 09:05:55	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:05:16	announced	2024-10-22: Updated project metadata.

10.1074/mcp.m113.028100;

Ischebeck T, Valledor L, Lyon D, Gingl S, Nagler M, Meij, ó, n M, Egelhofer V, Weckwerth W, Comprehensive cell-specific protein analysis in early and late pollen development from diploid microsporocytes to pollen tube growth. Mol Cell Proteomics, 13(1):295-310(2014) [pubmed]

submitter keyword: microspores, pollen tubes, development,tobacco, pollen

Till Ischebeck
contact affiliation	University of Münster, Institute of Plant Biology and Biotechnology (IBBP), Green Biotechnology, 48143 Münster, Germany
contact email	till.ischebeck@uni-muenster.de
lab head
Till Ischebeck
contact affiliation	University of Münster
contact email	tischeb@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045970
     1. Label: PRIDE project
     2. Name: Comprehensive cell-specific protein analysis in early and late pollen development from diploid microsporocytes to pollen tube growth