⮝ Full datasets listing

PXD045961

PXD045961 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOverexpress S-protein tagged mouse TBK1 protein and identify the TBK1-interacting proteins by mass spectrometry
DescriptionHEK293T cells were transfected with SFB-tagged mTBK1 or an empty vector for 24 h, the cells were lysed with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, 1 mM ethylenediaminetetraacetic acid) containing a complete protease inhibitor cocktail, followed by centrifugation at 16000xg for 10 min at 4 °C. The supernatants were subjected to immunoprecipitation using S-protein agarose (Millipore, Cat# 69704) for 3 h at 4 °C. Immunoprecipitates were washed with lysis buffer containing 150 mM NaCl three times and incubated with cell lysates from RAW 264.7 cells overnight at 4 °C. The immunoprecipitated complexes were washed three times again and separated by SDS-PAGE, and the gel was stained with Coomassie brilliant blue. The entire lane was cut into 2-mm gel slices, digested with Trypsin, and subjected to LC-MS/MS assays using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). The mass spectrometry data were analyzed using Thermo Proteome Discovery (Version2.3), and tandem mass spectra were searched against the UniProt- the UniProt Mus musculus database 2022.11.16.
HostingRepositoryiProX
AnnounceDate2023-10-27
AnnouncementXMLSubmission_2023-12-04_20:58:47.283.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJing Yang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-07 20:16:21ID requested
12023-10-07 20:16:39announced
22023-12-04 20:58:48announced2023-12-05: Update publication information.
Publication List
Lin Y, Yang J, Yang Q, Zeng S, Zhang J, Zhu Y, Tong Y, Li L, Tan W, Chen D, Sun Q, PTK2B promotes TBK1 and STING oligomerization and enhances the STING-TBK1 signaling. Nat Commun, 14(1):7567(2023) [pubmed]
Keyword List
submitter keyword: SFB-tagged mTBK1, RAW 264.7 cells, LC-MS/MS assays
Contact List
Qinmiao Sun
contact affiliationInstitute of Zoology, Chinese Academy of Sciences
contact emailqinmiaosun@ioz.ac.cn
lab head
Jing Yang
contact affiliationInstitute of Zoology, Chinese Academy of Sciences
contact emailyangjing22@ioz.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI