PXD045961
PXD045961 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Overexpress S-protein tagged mouse TBK1 protein and identify the TBK1-interacting proteins by mass spectrometry |
Description | HEK293T cells were transfected with SFB-tagged mTBK1 or an empty vector for 24 h, the cells were lysed with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, 1 mM ethylenediaminetetraacetic acid) containing a complete protease inhibitor cocktail, followed by centrifugation at 16000xg for 10 min at 4 °C. The supernatants were subjected to immunoprecipitation using S-protein agarose (Millipore, Cat# 69704) for 3 h at 4 °C. Immunoprecipitates were washed with lysis buffer containing 150 mM NaCl three times and incubated with cell lysates from RAW 264.7 cells overnight at 4 °C. The immunoprecipitated complexes were washed three times again and separated by SDS-PAGE, and the gel was stained with Coomassie brilliant blue. The entire lane was cut into 2-mm gel slices, digested with Trypsin, and subjected to LC-MS/MS assays using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). The mass spectrometry data were analyzed using Thermo Proteome Discovery (Version2.3), and tandem mass spectra were searched against the UniProt- the UniProt Mus musculus database 2022.11.16. |
HostingRepository | iProX |
AnnounceDate | 2023-10-27 |
AnnouncementXML | Submission_2023-12-04_20:58:47.283.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jing Yang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-07 20:16:21 | ID requested | |
1 | 2023-10-07 20:16:39 | announced | |
⏵ 2 | 2023-12-04 20:58:48 | announced | 2023-12-05: Update publication information. |
Publication List
Lin Y, Yang J, Yang Q, Zeng S, Zhang J, Zhu Y, Tong Y, Li L, Tan W, Chen D, Sun Q, PTK2B promotes TBK1 and STING oligomerization and enhances the STING-TBK1 signaling. Nat Commun, 14(1):7567(2023) [pubmed] |
Keyword List
submitter keyword: SFB-tagged mTBK1, RAW 264.7 cells, LC-MS/MS assays |
Contact List
Qinmiao Sun | |
---|---|
contact affiliation | Institute of Zoology, Chinese Academy of Sciences |
contact email | qinmiaosun@ioz.ac.cn |
lab head | |
Jing Yang | |
contact affiliation | Institute of Zoology, Chinese Academy of Sciences |
contact email | yangjing22@ioz.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |