PXD045960

PXD045960 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of serum extracellular vesicles from biliary tract infection patients to identify novel biomarkers
Description	Biliary tract infection (BTI) is a common abdominal disease and without proper management can even lead to bacteremia and further death. Although studies have focused on the initiation and mechanisms of this disease, diagnostic biomarkers for BTI are still lacking. Extracellular vesicles (EVs) are a kind of biological noano-particles that released from multiple types of cells reflected different physiological or pathological conditions. In the past decades, the development of EV-based diagnostic methods for many diseases is emerging. However, the utilization of EV-directed approaches for BTI diagnosis has not been well-investigated. To explore effective protein biomarkers for patients with BTI, we applied a label-free quantitative proteomic method, which is unbiased and high through-put, on the EVs from serum of control healthy donors or patients with BTI in this study. After analyzing the results, 62 novel proteins were unique in the BTI samples from the healthy controls. Moreover, 192 differentially expressed proteins were identified in EVs from patients with BTI when compared with those from healthy controls. To gain an overview on the functions of these proteins and the biological processes/pathways that the proteins are involved in, we performed GO and KEGG analysis by using this set of proteins an input. Importantly, some key pathways involved in fat acid processing were enriched as key pathways. Here we first idenfified biomarkers, Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and Crumbs homolog 3 (CRB3) presented significant different between BTI patients and healthy individuals. Clinical validation from plasma derived EVs samples that demonstrated its specificity to BTI. Our work applied an unbiased proteomic tool to identify CEACAM1 and CRB3 protein biomarkers in serum EVs may facilitate the development of diagnostic systems to identify patients with BTI.
HostingRepository	iProX
AnnounceDate	2023-10-07
AnnouncementXML	Submission_2024-03-11_01:23:01.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chih-Jung Chang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-07 20:14:31	ID requested
1	2023-10-07 20:14:51	announced
⏵ 2	2024-03-11 01:23:02	announced	2024-03-11: Update information.

Chang CJ, Huang YN, Lu YB, Zhang Y, Wu PH, Huang JS, Yang W, Chiang TY, Hsieh HS, Chung WH, Weng YC, Proteomic analysis of serum extracellular vesicles from biliary tract infection patients to identify novel biomarkers. Sci Rep, 14(1):5707(2024) [pubmed]

submitter keyword: Proteomics, Extracellular vesicles, Biliary tract infection, CEACAM1,CRB3

Chih-Jung Chang
contact affiliation	Xiamen Chang Gung Hospital
contact email	chan.chih.jung@gmail.com
lab head
Chih-Jung Chang
contact affiliation	Xiamen Chang Gung Hospital
contact email	chan.chih.jung@gmail.com
dataset submitter

iProX dataset URI