PXD045948

PXD045948 is an original dataset announced via ProteomeXchange.

Title	FGL1 IP-MS data in 293T cell lysates
Description	We immunoprecipitated exogenously expressed Myc-FGL1 using Anti-Myc-Tag monoclonal antibody (DIA-AN, Wuhan, China, Cat#2097) from 293T cells cocultured with tumor associated macrophages (TAMs) for 16 hr. No FGL1 protein was identified in protein lysates in which normal mouse IgG (Santa Cruz, California, USA, Cat#sc-2025) was added. Thus, protein lysates of 293T cells cocultured with TAMs added to mouse IgG were utilized as the negative control. A high-throughput technology, LC/MS (MS), was applied to identify the candidate interacting proteins of FGL1.
HostingRepository	iProX
AnnounceDate	2023-10-03
AnnouncementXML	Submission_2023-12-04_20:59:59.539.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiajia Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-06 23:39:38	ID requested
1	2023-10-06 23:40:01	announced
⏵ 2	2023-12-04 21:00:00	announced	2023-12-05: Update publication information.

Li JJ, Wang JH, Tian T, Liu J, Zheng YQ, Mo HY, Sheng H, Chen YX, Wu QN, Han Y, Liao K, Pan YQ, Zeng ZL, Liu ZX, Yang W, Xu RH, Ju HQ, The liver microenvironment orchestrates FGL1-mediated immune escape and progression of metastatic colorectal cancer. Nat Commun, 14(1):6690(2023) [pubmed]

submitter keyword: FGL1, Immunoprecipitation, Mass spectrometry, Coculture with tumor-associated macrophages

Huaiqiang Ju
contact affiliation	Sun Yat-sen University Cancer Center
contact email	juhq@sysucc.org.cn
lab head
Jiajia Li
contact affiliation	Sun Yat-sen University Cancer Center
contact email	lijj2@sysucc.org.cn
dataset submitter

iProX dataset URI