PXD045938

PXD045938 is an original dataset announced via ProteomeXchange.

Title	Exogenous pyruvate promotes gentamicin uptake to kill antibiotic-resistant Vibrio alginolyticus
Description	Elucidating antibiotic resistance mechanisms is necessary for developing novel therapeutic strategies. The increasing incidence of antibiotic-resistant Vibrio alginolyticus infection threatens both human health and aquaculture, but the mechanism has not been fully elucidated. Here, an isobaric tags for relative and absolute quantification (iTRAQ) functional proteomics analysis was performed on gentamicin-resistant V. alginolyticus (VA-RGEN) and a gentamicin-sensitive strain in order to characterize the global protein expression changes upon gentamicin resistance. Proteomics analysis demonstrated a global metabolic downshift in VA-RGEN, where the pyruvate cycle (the P cycle) was severely compromised. Exogenous pyruvate restored the P cycle activity, disrupting the redox state and increasing the membrane potential. It thereby potentiated gentamicin-mediated killing by approximately 3000- and 150-fold in vitro and in vivo, respectively. More importantly, bacterial gentamicin pharmacokinetics indicated that pyruvate enhanced gentamicin influx to a degree that exceeded the gentamicin expelled by the bacteria, increasing the intracellular gentamicin. Thus, our study suggests a metabolism-based approach to combating gentamicin-resistant V. algonolyticus, which paves the way for combating other types of antibiotic-resistant bacterial pathogens.
HostingRepository	iProX
AnnounceDate	2023-09-29
AnnouncementXML	Submission_2023-10-06_22:13:35.325.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kuangsu Fang
SpeciesList	scientific name: Vibrio alginolyticus; NCBI TaxID: 663;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-06 22:13:23	ID requested
⏵ 1	2023-10-06 22:13:35	announced

Dataset with its publication pending

submitter keyword: Antibiotic resistance, pyruvate cycle, gentamicin, proteomics, Vibrio alginolyticus

Bo Peng
contact affiliation	Sun Yat-sen University
contact email	pengb26@sysu.edu.cn
lab head
Kuangsu Fang
contact affiliation	Sun Yat-sen University
contact email	sufangkuang@126.com
dataset submitter

iProX dataset URI