PXD045936

PXD045936 is an original dataset announced via ProteomeXchange.

Title	Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents
Description	Heterotypic ubiquitin (Ub) chains have emerged as fundamental components in a wide range of cellular processes. The integrative identification of Ub-interacting proteins (readers) and Ub-modifying enzymes (writers and erasers) that selectively recognize and regulate heterotypic ubiquitination may provide crucial insights into these processes. In this study, we employed the bifunctional molecule-assisted (CAET) strategy to develop a new type of disulfide-bond-activated heterotypic Ub reagents, which allowed to enrich heterotypic Ub-interacting proteins and modifying enzymes simultaneously. The sequential release of readers which are non-covalently bound and writers or erasers which are covalently conjugated by using urea and reductant, respectively, combined with label-free quantitative (LFQ) MS indicated that these innovative heterotypic Ub tools would facilitate future investigations into functional roles played by heterotypic Ub chains.
HostingRepository	iProX
AnnounceDate	2023-09-28
AnnouncementXML	Submission_2024-05-21_00:48:11.434.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yicheng Weng
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue; methionine oxidation with neutral loss of 64 Da; acetylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-06 22:04:17	ID requested
⏵ 1	2024-05-21 00:48:12	announced

Cai H, Wu X, Mao J, Tong Z, Yan D, Weng Y, Zheng Q, Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents. Bioorg Chem, 145():107186(2024) [pubmed]

submitter keyword: Ubiquitin, chain type, chemical proteomics

Qingyun Zheng
contact affiliation	Shanghai Jiao Tong University
contact email	zheng_qy@sjtu.edu.cn
lab head
Yicheng Weng
contact affiliation	Shanghai Jiao Tong University
contact email	wengyc12@qq.com
dataset submitter

iProX dataset URI