PXD045936
PXD045936 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents |
Description | Heterotypic ubiquitin (Ub) chains have emerged as fundamental components in a wide range of cellular processes. The integrative identification of Ub-interacting proteins (readers) and Ub-modifying enzymes (writers and erasers) that selectively recognize and regulate heterotypic ubiquitination may provide crucial insights into these processes. In this study, we employed the bifunctional molecule-assisted (CAET) strategy to develop a new type of disulfide-bond-activated heterotypic Ub reagents, which allowed to enrich heterotypic Ub-interacting proteins and modifying enzymes simultaneously. The sequential release of readers which are non-covalently bound and writers or erasers which are covalently conjugated by using urea and reductant, respectively, combined with label-free quantitative (LFQ) MS indicated that these innovative heterotypic Ub tools would facilitate future investigations into functional roles played by heterotypic Ub chains. |
HostingRepository | iProX |
AnnounceDate | 2023-09-28 |
AnnouncementXML | Submission_2024-05-21_00:48:11.434.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yicheng Weng |
SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue; methionine oxidation with neutral loss of 64 Da; acetylated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-06 22:04:17 | ID requested | |
⏵ 1 | 2024-05-21 00:48:12 | announced |
Publication List
Cai H, Wu X, Mao J, Tong Z, Yan D, Weng Y, Zheng Q, Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents. Bioorg Chem, 145():107186(2024) [pubmed] |
Keyword List
submitter keyword: Ubiquitin, chain type, chemical proteomics |
Contact List
Qingyun Zheng | |
---|---|
contact affiliation | Shanghai Jiao Tong University |
contact email | zheng_qy@sjtu.edu.cn |
lab head | |
Yicheng Weng | |
contact affiliation | Shanghai Jiao Tong University |
contact email | wengyc12@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |