PXD045918

PXD045918 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of YqfB enzyme expressed in colorectal cancer cell line HCT116 using LC-MS/MS proteomics
Description	Gene-directed enzyme prodrug therapy is an emerging strategy for cancer treatment based on the delivery of a gene that encodes an enzyme that is able to convert a prodrug into a potent cytotoxin exclusively in target cancer cells. The aim of this study is to determine the capability of bacterial amidohydrolases to activate novel prodrugs and the effect such system has on the viability of eukaryotic cancer cells. HCT116 cancer cell line was subjected to LC-MS/MS proteomic analysis to confirm bacterial YqfB amidohydrolase expression at protein level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:26:46.046.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gediminas Plakys
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-05 13:19:10	ID requested
1	2024-01-26 08:13:06	announced
⏵ 2	2024-10-22 06:26:48	announced	2024-10-22: Updated project metadata.

Publication List

10.1371/journal.pone.0294696;
Preitakait, ė V, Barasa P, Au, č, ynait, ė A, Plakys G, Kopl, ū, nait, ė M, Zubavi, č, i, ū, t, ė S, Me, š, kys R, Bacterial amidohydrolases and modified 5-fluorocytidine compounds: Novel enzyme-prodrug pairs. PLoS One, 18(11):e0294696(2023) [pubmed]

10.1371/journal.pone.0294696;

Preitakait, ė V, Barasa P, Au, č, ynait, ė A, Plakys G, Kopl, ū, nait, ė M, Zubavi, č, i, ū, t, ė S, Me, š, kys R, Bacterial amidohydrolases and modified 5-fluorocytidine compounds: Novel enzyme-prodrug pairs. PLoS One, 18(11):e0294696(2023) [pubmed]

Keyword List

submitter keyword: LC-MSMS, HCT116,Colorectal cancer, YqfB

submitter keyword: LC-MSMS, HCT116,Colorectal cancer, YqfB

Contact List

Rolandas Meškys
contact affiliation	Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio 7, Vilnius, LT-10257, Lithuania
contact email	rolandas.meskys@bchi.vu.lt
lab head
Gediminas Plakys
contact affiliation	Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio 7, Vilnius, LT-10257, Lithuania
contact email	gediminas.plakys@gmail.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045918

PRIDE project URI

Repository Record List

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