PXD045907

PXD045907 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied with Fibrosis in Human Stroma
Description	Cornea transplantation is a widely performed procedure globally. However, storing donor corneas for extended periods in Optisol-GS storage medium at 4 ℃ can lead to reduced endothelial cell viability, causing severe stromal edema and opacification. This negatively impacts transplantation success. The corneal stroma, crucial for maintaining shape and transparency, was subjected to proteomic analysis after 7- or 14-day preservation. Among 1,545 identified proteins, 1,113 were quantifiable, with 255 significantly regulated in longer preservation. Proteins related to ocular surface immunomodulation decreased, while those involved in extracellular matrix reorganization and fibrosis increased after 14 days. The observed upregulation of extracellular matrix structural proteins and growth factor signaling suggests the occurrence of stromal fibrosis, potentially leading to compromised tissue clarity and vision impairments. This study provides novel insights into the impact of preservation duration on the corneal stroma from a proteomic perspective. Our findings may aid future research in developing long-term preservation techniques and enhancing the quality of preserved corneas, thereby maximizing their clinical usefulness.
HostingRepository	jPOST
AnnounceDate	2024-09-29
AnnouncementXML	Submission_2024-09-28_08:00:05.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miao-Hsia Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-04 20:23:34	ID requested
⏵ 1	2024-09-28 08:00:06	announced

Publication List

Wu PS, Liu HY, Wong TH, Lin JT, Hu FR, Lin MH, Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma. J Proteome Res, 22(12):3730-3741(2023) [pubmed]

Wu PS, Liu HY, Wong TH, Lin JT, Hu FR, Lin MH, Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma. J Proteome Res, 22(12):3730-3741(2023) [pubmed]

Keyword List

submitter keyword: corneal transplantation
corneal stroma
proteomics
LC-MS/MS analysis
ocular sur-face immunity
fibrosis

submitter keyword: corneal transplantation

corneal stroma

proteomics

LC-MS/MS analysis

ocular sur-face immunity

fibrosis

Contact List

Miao-Hsia Lin
lab head
Miao-Hsia Lin
contact affiliation	Department of Microbiology, College of Medicine, National Taiwan University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002340/