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PXD045907

PXD045907 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied with Fibrosis in Human Stroma
DescriptionCornea transplantation is a widely performed procedure globally. However, storing donor corneas for extended periods in Optisol-GS storage medium at 4 ℃ can lead to reduced endothelial cell viability, causing severe stromal edema and opacification. This negatively impacts transplantation success. The corneal stroma, crucial for maintaining shape and transparency, was subjected to proteomic analysis after 7- or 14-day preservation. Among 1,545 identified proteins, 1,113 were quantifiable, with 255 significantly regulated in longer preservation. Proteins related to ocular surface immunomodulation decreased, while those involved in extracellular matrix reorganization and fibrosis increased after 14 days. The observed upregulation of extracellular matrix structural proteins and growth factor signaling suggests the occurrence of stromal fibrosis, potentially leading to compromised tissue clarity and vision impairments. This study provides novel insights into the impact of preservation duration on the corneal stroma from a proteomic perspective. Our findings may aid future research in developing long-term preservation techniques and enhancing the quality of preserved corneas, thereby maximizing their clinical usefulness.
HostingRepositoryjPOST
AnnounceDate2024-09-29
AnnouncementXMLSubmission_2024-09-28_08:00:05.242.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMiao-Hsia Lin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-04 20:23:34ID requested
12024-09-28 08:00:06announced
Publication List
Wu PS, Liu HY, Wong TH, Lin JT, Hu FR, Lin MH, Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma. J Proteome Res, 22(12):3730-3741(2023) [pubmed]
Keyword List
submitter keyword: corneal transplantation
corneal stroma
proteomics
LC-MS/MS analysis
ocular sur-face immunity
fibrosis
Contact List
Miao-Hsia Lin
lab head
Miao-Hsia Lin
contact affiliationDepartment of Microbiology, College of Medicine, National Taiwan University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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