PXD045905
PXD045905 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of Purified Calpain-2 and Secondary Lipid Oxidation Products In-Vitro with LC-MS/MS and MALDI |
Description | Secondary lipid oxidation products modify and impact the function of proteins. Identifying where and how these products modify a protein's structure would allow for targeted approaches to mitigate or prevent this modification. Here, we purified calpain-2, a neutral calcium dependent cysteine protease, from porcine skeletal muscle and conducted in-vitro experiments using various concentrations of malondialdehyde, 2-hexenal, and 4-hydroxynonenal. We determined these secondary lipid oxidation products differentially impact calpain-2 proteolytic activity and autolysis. We identified modifications of malondialdehyde and hexenal on calpain-2 with LC-MS/MS approaches. We further evaluated the number of adduction sites on the catalytic and regulatory subunits of calpain-2 through MALDI-MS approaches. These data demonstrate that secondary lipid oxidation products can modify and differentially impact calpain-2. |
HostingRepository | MassIVE |
AnnounceDate | 2024-05-14 |
AnnouncementXML | Submission_2024-05-14_11:05:42.166.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Logan Johnson |
SpeciesList | scientific name: Sus scrofa; common name: pig; NCBI TaxID: 9823; |
ModificationList | Oxidation; S-carboxamidomethyl-L-cysteine; malondialdehyde lysine adduct (+54 amu); HNE; unknown modification |
Instrument | Orbitrap Eclipse; ultrafleXtreme |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-04 15:10:45 | ID requested | |
⏵ 1 | 2024-05-14 11:05:42 | announced |
Publication List
Logan G. Johnson, Chaoyu Zhai, Kitty Brown, Jessica E. Prenni, Mahesh N. Nair., Elisabeth Huff-Lonergan, and Steven M. Lonergan. Secondary Lipid Oxidation Products as Modulators of Calpain-2 Functionality In Vitro. J. Agric. Food Chem. 2024. doi: 10.1021/acs.jafc.4c00335. |
Keyword List
submitter keyword: in-vitro, malondialdehyde, hexenal, 4-hydroxynonenal, calpain-2 |
Contact List
Steven Lonergan | |
---|---|
contact affiliation | Iowa State University |
contact email | slonerga@iastate.edu |
lab head | |
Logan Johnson | |
contact affiliation | Iowa State University |
contact email | logan01@iastate.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093040/ |