PXD045901

PXD045901 is an original dataset announced via ProteomeXchange.

Title	Salivary Proteome Alterations in Medwakh Smokers Associated with Pathogenic Pathways: Implications for Youth Health
Description	Medwakh smoking has radically expanded among the youth in the Middle East and around the world. The rising popularity of medwakh/dokha usage is linked to the onset of several chronic illnesses including cardiovascular diseases and cancers. The present study aims to elucidate changes in the salivary proteome of medwakh smokers and explore the resultant pathological alterations in comparison to non-smokers. Statistical measurements reveal significant alterations in the abundance of 74 protein profiles in medwakh smokers compared to non-smokers. Medwakh smoking affected proteins with roles in cell anchorage and adhesion (FERMT3, CAPZA2, ACTN1, TLN1), immune responses, and inflammation (CFI, PSME2, S100A12, CHI3L1, HRG, RNASE2, ORM2), oxidative and anti-oxidative stress responses (NCF4, ERP 29, AKRA1, NADPH, NCF1B) and various cell metabolic processes (G6PD, HEXB, HK3, EEF1A1, PYGL, UGP2, EEF1A1). In our investigation, several novel proteins such as CFI, RNASE2, ORM2, NQO2, SULT1A1, TSN, and UBA1 were also discovered. These proteins play key roles in the initiation of various pathologies and are unique in medwakh smokers. The high significant increase in the abundance of involucrin suggests an alarming squamous cell differentiation in medwakh smokers. In addition, various pathways related to mitochondrial damage, oxidative burst, cell adhesion, migration, differentiation, and proliferation were significantly altered in medwakh smokers. The knowledge gained would help to elucidate the adverse effects of medwakh smoking, especially among adolescents, raising awareness of plausible health hazards, thereby improving the quality of life.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_08:34:05.764.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Giddey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-04 09:13:15	ID requested
⏵ 1	2025-05-06 08:34:06	announced

Aghila Rani KG, Soares NC, Rahman B, Giddey AD, Al-Hroub HM, Semreen MH, Al Kawas S, Medwakh smoking induces alterations in salivary proteins and cytokine expression: a clinical exploratory proteomics investigation. Clin Proteomics, 22(1):2(2025) [pubmed]

10.1186/s12014-024-09520-6;

submitter keyword: Medwakh, Human, Saliva, Smoking, Youth

Mohammed Harb Semreen
contact affiliation	1 - College of Pharmacy, University of Sharjah, Sharjah 2 - Sharjah Institute for Medical Research, University of Sharjah, Sharjah
contact email	msemreen@sharjah.ac.ae
lab head
Alexander Giddey
contact affiliation	University of Cape Town
contact email	gddale001@myuct.ac.za
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD045901

PRIDE project URI

• PRIDE
  1. PXD045901
     1. Label: PRIDE project
     2. Name: Salivary Proteome Alterations in Medwakh Smokers Associated with Pathogenic Pathways: Implications for Youth Health