PXD045886 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome birthdating reveals age-selectivity of protein ubiquitination |
Description | Within a cell, proteins have distinct and highly variable half-lives. As a result, molecular ages of proteins can range from seconds to years. How the age of a protein influences its environmental interactions is a largely unexplored area of biology. To investigate the age-selectivity of cellular pathways, we developed a methodology termed “proteome birthdating” that barcodes proteins based on their time of synthesis. We show that this approach provides accurate measurements of protein turnover kinetics without the requirement for multiple kinetic time points. As a first use case of the birthdated proteome, we investigated the age distribution of the human ubiquitinome. Our results indicate that the vast majority of ubiquitinated proteins in a cell consist of newly synthesized proteins and that these young proteins constitute the bulk of the degradative flux through the proteasome. Rapidly ubiquitinated proteins are enriched in cytosolic proteins and subunits of protein complexes. Conversely, proteins destined for the secretory pathway and vesicular transport have older ubiquitinated populations. Our data also identified a smaller subset of very old ubiquitinated cellular proteins that do not appear to be targeted to the proteasome for rapid degradation. Together, our data provide an age census of the human ubiquitinome and establish proteome birthdating as a methodology for investigating the protein age-selectivity of diverse cellular pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-18 |
AnnouncementXML | Submission_2024-06-17_20:23:33.292.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Meadow |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-03 16:09:07 | ID requested | |
⏵ 1 | 2024-06-17 20:23:33 | announced | |
Publication List
Keyword List
submitter keyword: Proteostasis, Aging,Protein turnover |
Proteomics, Mass spectrometry (MS), Ubiquitin Proteasome System (UPS) |
Contact List
Sina Ghaemmaghami |
contact affiliation | Department of Biology, University of Rochester, NY, 14627, USA University of Rochester Mass Spectrometry Resource Laboratory, NY, 14627, USA |
contact email | sina.ghaemmaghami@rochester.edu |
lab head | |
Michael Meadow |
contact affiliation | University of Rochester |
contact email | Michael_meadow@urmc.Rochester.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045886
- Label: PRIDE project
- Name: Proteome birthdating reveals age-selectivity of protein ubiquitination