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PXD045886

PXD045886 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome birthdating reveals age-selectivity of protein ubiquitination
DescriptionWithin a cell, proteins have distinct and highly variable half-lives. As a result, molecular ages of proteins can range from seconds to years. How the age of a protein influences its environmental interactions is a largely unexplored area of biology. To investigate the age-selectivity of cellular pathways, we developed a methodology termed “proteome birthdating” that barcodes proteins based on their time of synthesis. We show that this approach provides accurate measurements of protein turnover kinetics without the requirement for multiple kinetic time points. As a first use case of the birthdated proteome, we investigated the age distribution of the human ubiquitinome. Our results indicate that the vast majority of ubiquitinated proteins in a cell consist of newly synthesized proteins and that these young proteins constitute the bulk of the degradative flux through the proteasome. Rapidly ubiquitinated proteins are enriched in cytosolic proteins and subunits of protein complexes. Conversely, proteins destined for the secretory pathway and vesicular transport have older ubiquitinated populations. Our data also identified a smaller subset of very old ubiquitinated cellular proteins that do not appear to be targeted to the proteasome for rapid degradation. Together, our data provide an age census of the human ubiquitinome and establish proteome birthdating as a methodology for investigating the protein age-selectivity of diverse cellular pathways.
HostingRepositoryPRIDE
AnnounceDate2024-06-18
AnnouncementXMLSubmission_2024-06-17_20:23:33.292.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Meadow
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitinylated lysine
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-03 16:09:07ID requested
12024-06-17 20:23:33announced
Publication List
10.1016/J.MCPRO.2024.100791;
Keyword List
submitter keyword: Proteostasis, Aging,Protein turnover
Proteomics, Mass spectrometry (MS), Ubiquitin Proteasome System (UPS)
Contact List
Sina Ghaemmaghami
contact affiliationDepartment of Biology, University of Rochester, NY, 14627, USA University of Rochester Mass Spectrometry Resource Laboratory, NY, 14627, USA
contact emailsina.ghaemmaghami@rochester.edu
lab head
Michael Meadow
contact affiliationUniversity of Rochester
contact emailMichael_meadow@urmc.Rochester.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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