PXD045885

PXD045885 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of vitreous in WT and CD11b KO mice.
Description	Our data support that following optic nerve crush injury, ocular infiltration of immune cells causes damage to the retinal vasculature and compromises the integrity of the blood-retina barrier (BRB). Functional ablation of integrin alphaM (CD11b) attenuates immune cell trafficking, protects the BRB, and strongly enhances β-glucan-elicited retinal ganglion cells axon regeneration. Here, we performed an unbiased analysis of the vitreal proteome; we harvested vitreous humor from WT and Itgam-/- mice, β-glucan treated after optic nerve injury, and used mass spectrometry-based proteomics for differential analysis of the WT and Itgam-/- vitreous proteome (label free and tandem mass tagging).
HostingRepository	PRIDE
AnnounceDate	2026-08-05
AnnouncementXML	Submission_2026-08-05_08:56:06.768.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Oses-Prieto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-03 15:54:11	ID requested
⏵ 1	2026-08-05 08:56:14	announced

Passino R, Finneran MC, Hafner H, Feng Q, Huffman LD, Zhao XF, Johnson CN, Kawaguchi R, Oses-Prieto JA, Burlingame AL, Geschwind DH, Benowitz LI, Giger RJ, Neutrophil-inflicted vasculature damage suppresses immune-mediated optic nerve regeneration. Cell Rep, 43(3):113931(2024) [pubmed]

10.1016/j.celrep.2024.113931;

submitter keyword: Mouse, TMT, vitreous, Itgam., CD11b, label free

Roman J Giger
contact affiliation	Department of Cell and Developmental Biology, Neuroscience Graduate Program, Department of Neurology University of Michigan Medical School, Ann Arbor, MI 48109-2200
contact email	rgiger@umich.edu
lab head
Juan Oses-Prieto
contact affiliation	UCSF
contact email	joses@cgl.ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD045885

PRIDE project URI

• PRIDE
  1. PXD045885
     1. Label: PRIDE project
     2. Name: Proteomic analysis of vitreous in WT and CD11b KO mice.