PXD045883

PXD045883 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	DIA-MS proteomics of untreated and TAT-Cx43266-283-treated G166 GSCs
Description	Glioblastoma (GBM) is the most frequent and aggressive primary brain cancer. Our studies have shown that the Src inhibitor, TAT-Cx43266-283, exerts antitumor effects in different preclinical models of GBM, including fresh specimens from GBM patients, and enhances survival in glioma-bearing mice. Because addressing TAT-Cx43266-283 mechanism of action is essential to translate these results to a clinical setting, in this study we carried out an unbiased proteomic approach in human glioblastoma stem cells (GSCs). Data-independent acquisition mass spectrometry proteomics allowed the identification and quantification of 6,561 proteins, of which 190 were modified by TAT-Cx43266-283. Our results are consistent with the inhibition of Src as the mechanism of action of TAT-Cx43266-283 and unveils additional crucial proteins. Altogether, this study expands the knowledge about the mechanism of action of TAT-Cx43266-283, providing a rationale for therapy combination and supporting its use in GBM clinical trials.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:13.827.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara G. Pelaz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-03 12:18:21	ID requested
1	2024-06-21 23:38:26	announced
⏵ 2	2024-10-22 06:46:15	announced	2024-10-22: Updated project metadata.

Publication List

Pelaz SG, Flores-Hern, á, ndez R, Vujic T, Schvartz D, Á, lvarez-V, á, zquez A, Ding Y, Garc, í, a-Vicente L, Belloso A, Talaver, ó, n R, S, á, nchez JC, Tabernero A, in glioblastoma. Transl Res, 272():95-110(2024) [pubmed]
10.1016/j.trsl.2024.06.001;

Pelaz SG, Flores-Hern, á, ndez R, Vujic T, Schvartz D, Á, lvarez-V, á, zquez A, Ding Y, Garc, í, a-Vicente L, Belloso A, Talaver, ó, n R, S, á, nchez JC, Tabernero A, in glioblastoma. Transl Res, 272():95-110(2024) [pubmed]

10.1016/j.trsl.2024.06.001;

Keyword List

submitter keyword: glioblastoma GSCs DIA-MS Src-inhibitor

submitter keyword: glioblastoma GSCs DIA-MS Src-inhibitor

Contact List

Arantxa Tabernero
contact affiliation	Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Spain
contact email	ataber@usal.es
lab head
Sara G. Pelaz
contact affiliation	Department of Physiology, University of California Los Angeles (UCLA), USA
contact email	saragpelaz@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD045883
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD045883

PRIDE project URI

Repository Record List

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