PXD045883 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DIA-MS proteomics of untreated and TAT-Cx43266-283-treated G166 GSCs |
Description | Glioblastoma (GBM) is the most frequent and aggressive primary brain cancer. Our studies have shown that the Src inhibitor, TAT-Cx43266-283, exerts antitumor effects in different preclinical models of GBM, including fresh specimens from GBM patients, and enhances survival in glioma-bearing mice. Because addressing TAT-Cx43266-283 mechanism of action is essential to translate these results to a clinical setting, in this study we carried out an unbiased proteomic approach in human glioblastoma stem cells (GSCs). Data-independent acquisition mass spectrometry proteomics allowed the identification and quantification of 6,561 proteins, of which 190 were modified by TAT-Cx43266-283. Our results are consistent with the inhibition of Src as the mechanism of action of TAT-Cx43266-283 and unveils additional crucial proteins. Altogether, this study expands the knowledge about the mechanism of action of TAT-Cx43266-283, providing a rationale for therapy combination and supporting its use in GBM clinical trials. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:46:13.827.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sara G. Pelaz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-03 12:18:21 | ID requested | |
1 | 2024-06-21 23:38:26 | announced | |
⏵ 2 | 2024-10-22 06:46:15 | announced | 2024-10-22: Updated project metadata. |
Publication List
Pelaz SG, Flores-Hern, á, ndez R, Vujic T, Schvartz D, Á, lvarez-V, á, zquez A, Ding Y, Garc, í, a-Vicente L, Belloso A, Talaver, ó, n R, S, á, nchez JC, Tabernero A, in glioblastoma. Transl Res, 272():95-110(2024) [pubmed] |
10.1016/j.trsl.2024.06.001; |
Keyword List
submitter keyword: glioblastoma GSCs DIA-MS Src-inhibitor |
Contact List
Arantxa Tabernero |
contact affiliation | Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Spain |
contact email | ataber@usal.es |
lab head | |
Sara G. Pelaz |
contact affiliation | Department of Physiology, University of California Los Angeles (UCLA), USA |
contact email | saragpelaz@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045883
- Label: PRIDE project
- Name: DIA-MS proteomics of untreated and TAT-Cx43266-283-treated G166 GSCs