PXD045877

PXD045877 is an original dataset announced via ProteomeXchange.

Title	The Greater Celandine: Identification and characterization of a novel antimicrobial peptide sequence from Chelidonium majus
Description	A highly abundant, cysteine-rich peptide, deemed CM-AMP1, is characterized through multiple mass spectrometric approaches. Complimentary CID and EAD fragmentation types are used to gain full sequence coverage of the full-length peptide. CM-AMP1 shares little sequence similarity to any proteins in publicly available databases, highlighting the novelty of its cysteine landscape and core motif. The presence of three disulfide bonds in the native peptide confers proteolytic stability, and antimicrobial activity is greatly decreased upon alkylation of the cysteine residues. Synthetic variants of CM-AMP1 are used to confirm activity of the full-length sequence and the core motif. To assess the biological impact, E. coli was grown in a sub-lethal concentration of CM-AMP1 and quantitative proteomics was used to identify proteins produced by the bacteria under stress, ultimately suggesting a membrane lytic antimicrobial mechanism of action. This study integrates multiple analytical methods for molecular and biological characterization of a unique antimicrobial peptide identified from C. majus.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:15:24.768.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Chelidonium majus; NCBI TaxID: 71251;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	SCIEX instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-03 06:52:49	ID requested
⏵ 1	2024-10-08 11:15:25	announced

Dataset with its publication pending

submitter keyword: Celandine, antimicrobial response, proteomics,Natural products, antimicrobial peptide

Leslie Hicks
contact affiliation	UNC - Chapel Hill
contact email	lmhicks@unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045877
     1. Label: PRIDE project
     2. Name: The Greater Celandine: Identification and characterization of a novel antimicrobial peptide sequence from Chelidonium majus