PXD045873

PXD045873 is an original dataset announced via ProteomeXchange.

Title	Candida albicans biofilm extracellular vesicles deliver candidalysin to epithelial cell membranes and induce host cell responses
Description	Extracellular vesicles (EVs) are heterogeneous particles encapsulated with a phospholipid bilayer membrane. EVs have evolved diverse biological functions, serving mainly as prominent mediators and regulators of cell-cell communication. Fungal EVs contain numerous factors involved in a diverse array of biological functions including biogenesis of extracellular matrix proteins and saccharides, antifungal resistance, and pathogenesis. This study investigated whether candidalysin, a peptide toxin that drives C. albicans infections, is present in EVs derived from C. albicans biofilms. We found that those EVs contain candidalysin at low levels and can permeabilize planar lipid bilayers membranes in a dose-dependent manner but are unable to damage oral epithelial cells (OECs). Application of EVs to OECs induced cytokine responses but did not activate MAPK signalling. Notably, EVs obtained from biofilms cultured for 24 h and 48 h exhibited differences in cargo composition and their ability to activate OECs. This study suggests that secreted EVs can serve as a toxin delivery system during C. albicans infection but also possess different functions depending on their maturity.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:05:31.896.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045873
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Greg Sabat
SpeciesList	scientific name: Candida albicans SC5314; NCBI TaxID: 237561;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-03 03:33:58	ID requested
⏵ 1	2025-05-25 16:05:32	announced

10.6019/PXD045873;

10.1128/iai.00404-24;

Lee S, Tsavou A, Zarnowski R, Pforte R, Allert S, Kr, ü, ger T, Kniemeyer O, Brakhage AA, Bui TTT, Andes DR, Richardson JP, Hube B, Naglik JR, biofilm extracellular vesicles deliver candidalysin to epithelial cell membranes and induce host cell responses. Infect Immun, 93(5):e0040424(2025) [pubmed]

submitter keyword: Candida albicans, biofilm, vesicles

David Andes
contact affiliation	Medical Microbiology and Immunology, Division of Infectious Disease
contact email	dra@medicine.wisc.edu
lab head
Greg Sabat
contact affiliation	University of Wisconsin
contact email	gsabat@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD045873

PRIDE project URI

• PRIDE
  1. PXD045873
     1. Label: PRIDE project
     2. Name: Candida albicans biofilm extracellular vesicles deliver candidalysin to epithelial cell membranes and induce host cell responses