PXD045869

PXD045869 is an original dataset announced via ProteomeXchange.

Title	In-Gel Digest to identify Aspergillus fumigatus protein antigens in severe equine asthma (SEA)
Description	Aspergillus fumigatus (Asp f) has been described as a major extrinsic stimulus of SEA and is a common mold species in hay. Aiming to identify disease-relevant antigens, we analyzed Asp f as a source, in an immuno-proteomics approach on two-dimensional immunoblots. For 21 of 289 spots serum Ig binding was different between asthmatic and healthy horses’ sera. Proteins were in-gel digested and tryptic peptides extracted from the 21 spots of interest, analyzed by liquid chromatography-mass spectrometry and eight identified and prioritized candidate antigens were expressed as recombinant proteins.
HostingRepository	PanoramaPublic
AnnounceDate	2024-02-13
AnnouncementXML	Submission_2024-02-13_17:12:55.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniela Volke
SpeciesList	scientific name: Aspergillus fumigatus; NCBI TaxID: 746128;
ModificationList	Carbamidomethyl; Oxidation
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-02 20:46:07	ID requested
⏵ 1	2024-02-13 17:12:55	announced

Jentsch M-C, Lübke S, Schrödl W, Volke D, Krizsan A, Hoffmann R, Kaiser-Thom S, Gerber V, Marti E, Wagner B and Schnabel CL (2024) Immunoproteomics enables broad identification of new Aspergillus fumigatus antigens in severe equine asthma. Front. Immunol. 15:1347164. doi: 10.3389/fimmu.2024.1347164

submitter keyword: recurrent airway obstruction, COPD, proteomics, immunoglobulin isotype, serum, antibody, immunoblot

Ralf Hoffmann
contact affiliation	Universität Leipzig/BBZ
contact email	ralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliation	Universität Leipzig/BBZ
contact email	daniela.volke@bbz.uni-leipzig.de
dataset submitter

Panorama Public dataset URI