PXD045866

PXD045866 is an original dataset announced via ProteomeXchange.

Title	Etomoxir as a Promiscuous Chemoproteomic Probe.
Description	Primary hepatocytes from WT or Pex5KO mouse livers were treated with 100uM clickable etomoxir analogues and the proteins bound to etomoxir were pulled down and identified. The small molecule inhibitor etomoxir has been used for many years as a specific inhibitor of Cpt1 but these data show that etomoxir binds a large array of proteins and is not appropriate as a tool for evaluating the biological effects of fatty acid oxidation. Primary hepatocytes were treated with 100uM clickable etomoxir analogues and the proteins bound to etomoxir were pulled down and identified. The small molecule inhibitor etomoxir has been used for many years as a specific inhibitor of Cpt1 but these data show that etomoxir binds a large array of proteins and is not appropriate as a tool for evaluating the biological effects of fatty acid oxidation.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_03:57:59.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wolfgang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-02 13:43:45	ID requested
⏵ 1	2024-10-17 03:58:00	announced

Choi J, Smith DM, Lee YJ, Cai D, Hossain MJ, O'Connor TJ, Deme P, Haughey NJ, Scafidi S, Riddle RC, Wolfgang MJ, Etomoxir repurposed as a promiscuous fatty acid mimetic chemoproteomic probe. iScience, 27(9):110642(2024) [pubmed]

10.1016/j.isci.2024.110642;

submitter keyword: Liver, Fatty Acid Oxidation, Cpt1, Etomoxir

Michael J. Wolfgang
contact affiliation	The Johns Hopkins University School of Medicine, Baltimore, Maryland, Department of Biological Chemistry and Department of Pharmacology and Molecular Sciences
contact email	mwolfga1@jhmi.edu
lab head
Michael Wolfgang
contact affiliation	Johns Hopkins University School of Medicine
contact email	mwolfga1@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD045866

PRIDE project URI

• PRIDE
  1. PXD045866
     1. Label: PRIDE project
     2. Name: Etomoxir as a Promiscuous Chemoproteomic Probe.