PXD045865

PXD045865 is an original dataset announced via ProteomeXchange.

Title	Extracellular Signals Induce Dynamic ER Remodeling through TAT1-dependent Microtubule Acetylation
Description	Dynamic changes in the endoplasmic reticulum (ER) morphology are central to maintaining cellular homeostasis. Microtubules (MT) facilitate the continuous remodeling of the ER network into sheets and tubules by coordinating with many ER-shaping protein complexes, although how this process is controlled by extracellular signals remains unknown. Here we report that TAK1, a kinase responsive to various growth factors and cytokines including TGF- and TNF-, triggers ER tubulation by activating TAT1, an MT-acetylating enzyme that enhances ER-sliding. We show that this TAK1/TAT1-dependent ER remodeling promotes cell survival by actively downregulating BOK, an ER membrane-associated proapoptotic effector. While BOK is normally protected from degradation when complexed with IP3R, it is rapidly degraded upon their dissociation during the ER sheets-to-tubules conversion. These findings demonstrate a distinct mechanism of ligand-induced ER remodeling and suggest that the TAK1/TAT1 pathway may be a key target in ER stress and dysfunction.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-15_23:48:08.701.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045865
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Langlais
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-02 12:50:02	ID requested
⏵ 1	2024-06-15 23:48:09	announced

10.6019/PXD045865;

10.1016/j.neo.2024.101003;

Ortiz HR, Cruz Flores P, Podgorski J, Ramonett A, Ahmed T, Hempel N, Charest PG, Ellis NA, Langlais PR, Montfort WR, Mythreye K, Kumar S, Lee NY, TAT1-dependent microtubule acetylation. Neoplasia, 53():101003(2024) [pubmed]

submitter keyword: BOK, aTAT1, Microtubules, TAK1,Endoplasmic Reticulum

Nam Lee
contact affiliation	University of Arizona College of Medicine - Tucson
contact email	namlee@arizona.edu
lab head
Paul Langlais
contact affiliation	University of Arizona
contact email	langlais@deptofmed.arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045865
     1. Label: PRIDE project
     2. Name: Extracellular Signals Induce Dynamic ER Remodeling through TAT1-dependent Microtubule Acetylation