PXD045854

PXD045854 is an original dataset announced via ProteomeXchange.

Title	The mitotic STAG3-cohesin defines male germline nucleome
Description	Cohesin is a tetrameric protein complex with a ring-like structure that entraps DNA to ensure sister chromatid cohesion and higher-order chromatin organization.  The mechanism by which cohesin regulates chromatin structure and gene expression remains a fundamental question.  In multicellular organisms, germ cells are unique in that they tailor chromatin organization toward generating totipotency.  As part of this program, spermatogonial stem cells (SSCs) in mammals, the source for male gametes, acquire distinctive chromatin organization with exceptionally weak insulation, but the underlying mechanism is unknown.  Here, we show that STAG3, exclusively known to form meiotic cohesins, contributes to a mitotic cohesin defining nucleome programming during male germline development.  With slower loop extrusion and shorter chromatin residence, STAG3–cohesin attenuates topologically associating domains (TADs) demarcated by CCCTC-binding factor (CTCF), re-wires both enhancer–promoter and Polycomb-mediated repressive interactions, and creates finer and strengthened compartments, establishing a characteristic SSC nucleome.  Moreover, STAG3–cohesin controls the balance between SSC self-renewal and differentiation for spermatogenesis.  Notably, mitotic STAG3–cohesin is also highly expressed in human B cells and potentially involved in B-cell malignancies.  Thus, mitotic STAG3–cohesin with its unique loop extrusion property elucidates a principle for male germline nucleome programming and, more broadly, may contribute to human malignancy.
HostingRepository	jPOST
AnnounceDate	2025-07-07
AnnouncementXML	Submission_2025-07-06_18:06:31.310.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kosuke Ogata
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Acetyl; Acetyl; TMTpro; TMTpro
Instrument	instrument; instrument

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-02 07:29:27	ID requested
⏵ 1	2025-07-06 18:06:31	announced

Dataset with its publication pending

submitter keyword: cohesin, STAG3, loop extrusion, male germ cells, spermatogonial stem cells, insulation, compartments, nucleome programming

Mitinori Saitou
lab head
Kosuke Ogata
contact affiliation	Kyoto university
dataset submitter

jPOST dataset URI

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