PXD045849

PXD045849 is an original dataset announced via ProteomeXchange.

Title	Isolation and characterization of extracellular vesicles from Arabidopsis thaliana cell culture and investigation of the specificities of their biogenesis
Description	Over the past decade, membrane vesicles, known as exosomes, have garnered significant attention due to their potential as natural nanocarriers. Recently, it was discovered that plants also release extracellular vesicles (EVs). Yet, our understanding of EV production in plant cell culture remains limited. In this study, we used callus culture of the model plant Arabidopsis thaliana to isolate and comprehensively characterize EVs emanating from cultivated cells. Our findings reveal that the quantity of EVs in calli was significantly lower than in the plant's apoplastic fluid. This discrepancy is linked to the transcriptional repression of the endosomal sorting complex required for transport (ESCRT) genes. While salicylic acid elevated the expression of ESCRT components, it did not boost EV production. Remarkably, EVs from calli encompass proteins pivotal for cell wall biogenesis and defense mechanisms, as well as microRNAs consistent with those in intact plants. This indicates that plant cell cultures might be a viable source of EVs reflecting the properties of the parent plant species. Nevertheless, additional research is crucial to pinpoint the optimal conditions for efficient EV production.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:37:53.946.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045849
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dmitriy Bulgakov
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-02 06:39:47	ID requested
⏵ 1	2024-01-26 07:37:54	announced

10.3390/plants12203604;

Yugay Y, Tsydeneshieva Z, Rusapetova T, Grischenko O, Mironova A, Bulgakov D, Silant'ev V, Tchernoded G, Bulgakov V, Shkryl Y, Cell Culture and Investigation of the Specificities of Their Biogenesis. Plants (Basel), 12(20):(2023) [pubmed]

10.6019/PXD045849;

submitter keyword: exosomes

nanoparticles

membrane trafficking

callus culture

salicylic acid

Yuri Nikolaevich Shkryl
contact affiliation	Head of the Laboratory of bionanotechnology and biomedicine, Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia
contact email	yn80@mail.ru
lab head
Dmitriy Bulgakov
contact affiliation	Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok-22, 690022, Russia
contact email	bulgakov-dv@mail.ru
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045849

PRIDE project URI

• PRIDE
  1. PXD045849
     1. Label: PRIDE project
     2. Name: Isolation and characterization of extracellular vesicles from Arabidopsis thaliana cell culture and investigation of the specificities of their biogenesis